白柄肉齿菌Sarcodon leucopus多酚生物碱提取物的体外抗氧化及降血糖活性

为探讨西藏白柄肉齿菌Sarcodon leucopus子实体多酚生物碱提取物（PAE）的抗氧化和降糖活性,本研究考察了PAE对DPPH自由基清除能力、细胞内抗氧化能力（CAA）和糖尿病关键酶（大鼠小肠来源麦芽糖酶、蔗糖酶和α-淀粉酶）活性的抑制能力。结果表明,PAE具有一定的抗氧化活性,对DPPH自由基的半数抑制浓度（IC₅₀）为(18.12±1.09)μg/mL,对细胞内抗氧化能力表现为CAA₅₀=(13.60±0.13)μg/mL;PAE对糖尿病关键酶的抑制活性有一定选择性,对麦芽糖酶和蔗糖酶具有抑制作用,并呈剂量效应关系,其IC₅₀分别(60.86±4.50)μg/mL和(64.60±4.53)μg/mL,而对α-淀粉酶抑制作用极低。研究结果表明PAE有潜在的抗氧化及降血糖活性,具有开发为安全高效的天然降血糖功能食品和药物的潜力。

Antioxidant and hypoglycemic activities of polyphenolic alkaloid-enriched extract from Sarcodon leucopus

To investigate the antioxidant and hypoglycemic activities of polyphenolic alkaloid-enriched extract(PAE)from Sarcodon leucopus fruiting bodies in Tibet,the DPPH free radical scavenging activities,intracellular antioxidant capacity(CAA)and inhibitory effects on key enzymes linked to diabetes(maltase,sucrase,andα-amylase activity from rat small intestine)were tested.The results showed that PAE had antioxidant activity in a dose-dependent manner,and the IC50 value of DPPH free radicals was(18.12±1.09)μg/mL,and CAA50 value of the intracellular antioxidant capacity was(13.60±0.13)μg/mL.PAE also showed selective Inhibitory effect on key enzymes relevant to diabetes with a dose-dependent effect.IC50 values of maltase and sucrase were(60.86±4.50)μg/mL and(64.60±4.53)μg/mL,respectively,while the inhibitory effect onα-amylase was extremely low.PAE of S.leucopus was found to possess antioxidant and hypoglycemic activities,which was hopeful of developing safe and active ingredient of functional foods or medicines for preventing or alleviating diabetes.