中国弯颈霉新种及产环孢菌素的研究

对云南省迪庆藏族自治州产的冬虫夏草 Cordyceps sinensis(Berk.)Sacc.进行分离获一纯种,经培养鉴定确为弯颈霉属 Tolypocladium w.Gams 一新种,命名为中国弯颈霉 To-lypocladium sinense c.L.Li sp.nov.。它在几种琼脂培养基上生长形成瓶梗及瓶梗孢于,瓶梗基部呈球形或椭圆形膨大,瓶颈细长而弯曲,瓶梗孢子球形至卵形。其形态特征及生活习性与弯颈霉属其它种类显然不同,故定为一新种。它在蛋白胨葡萄糖培养液中,温度26℃,pH5.2,摇瓶振荡培养12天。发酵液用乙酸乙酯萃取,减压蒸馏,从每升发酵液中获50—80mg 环孢菌素粗品。对几种半知真菌显示了明显的拮抗作用。

A STUDY OF TOLYPOCLADIUM SINENSE C.L.LI.SP.NOV AND CYCLOSPORIN PRODUCTION

Fresh Contaminate specimens of Cordyceps sinensis (Berk.) Sacc.col- lected from Diqing Tibeten Austonompus Prefecture,Yunnan province,the contamina- tion were isolated and cultivated to obtain a pure species.It was identified as a new species of Tolypocladium namely Tolypocladlum sinanse C.L.Li.sp.nov.It forms phialides and phialospores in several agar media,the base of phialides are spherical or elliptical and terminated by slender,curved neck bearing globose to ovoid phialos- pores.The morphological characteristics of the new species are given in comparison with other species of Tolypocladium,it is obviously different from other species of Tolypocladium in shape and size of phialospores as well as phialides,also hosts and habitats,therefore we consider Tolypocladium sinense as a new species The new species is cultivated by submerged culture in liquid medium composed of peptone,glucose,inorganic salt etc,at 26℃,pH5.2,on shakers for 12days.The fermentation broth was extracted with an equal volume of acetic ether and the orga niclayer was separated and evaporated under reduced pressure.In this way 1 liter of fermentation broth yield approximately 50—80 mg of crude materal of cyclosporin. The crude materal exhibit antifungal activity for strains of some imperfect fungi.