| 不同氮形态对AM真菌孢子萌发和菌丝生长的影响 |
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| 引用本文： | 朱红惠,姚青,龙良坤,羊宋贞.不同氮形态对AM真菌孢子萌发和菌丝生长的影响[J].菌物学报,2004,23(4):590-595. |
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| 作者姓名： | 朱红惠 姚青 龙良坤 羊宋贞 |
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| 作者单位： | 1. 广东省微生物研究所广东省菌种保藏与应用重点实验室,广州,510070
2. 华南农业大学园艺学院,广州,510642 |
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| 基金项目： | 国家自然科学基金(30370033，30200006)，广东省科学院优秀人才基金资助项目 |
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| 摘 要： | 以Long-Ashton培养基为基础,采用NH4+-N、NO3--N和NH4+-NO3--N 3种氮源,研究了不同氮形态对AM真菌孢子萌发和菌丝生长的影响。结果表明,孢子的萌发速度在NO3--N和NH4+-NO3--N培养基上先快后慢,而NH4+-N培养基上先慢后快,但最终三者的萌发率没有差异,均达到50%左右;菌丝生长对3种氮形态的反应存在显著差异,NO3--N和NH4+-NO3--N培养基上的菌丝生长速率和长度大于NH4+-N培养基上的相应值。本试验表明,与NO3--N和NH4+-NO3--N 相比,NH4+-N虽然没有降低AM真菌孢子的萌发率,但是能够抑制萌发菌丝的生长。
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| 关 键 词： | Gigasporamargarita 硝态氮 氨态氮 萌发率 菌丝生长速率 |
| 文章编号： | 1672-6472(2004)04-0590-0595 |
| 修稿时间： | 2004年4月8日 |
INFLUENCE OF DIFFERENT N FORMS ON SPORE GERMINATION AND HYPHAL GROWTH OF AM FUNGUS |
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| Authors: | ZHU Hong-Hui YAO Qing LONG Liang-Kun YANG Sonng-Zhen |
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| Institution: | ZHU Hong-Hui1 YAO Qing2 LONG Liang-Kun1 YANG Sonng-Zhen1 |
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| Abstract: | Influence of different N forms on spore germination and hyphal growth of AM fungus, Gigaspora margarita, were investigated on Long-Ashton medium. Results indicated that spore germination was fast at early stage and slow at late stage on NO3--N and NH4 -NO3--N mediums, but just the reverse on NH4 -N medium. No difference was detected among spore germination rates on three mediums at finial stage, all reaching about 50%. Significant difference was observed among the hyphal growths on three mediums. Extending rate and length of germ hyphae were higher on NO3--N and NH4 -NO3--N mediums than on NH4 -N medium. This experiment demonstrated that NH4 -N didnt reduce AM fungal spore germination, but inhibited the hyphal growth, as compared with NO3--N and NH4 -NO3--N. |
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| Keywords: | Gigaspora margarita NO3--N NH4 -N germination rate hyphal extending rate |
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