| 鸡菌的液体培养及其多糖物质研究 |
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| 引用本文： | 朱必凤,马海燕,赵发清,傅明辉.鸡菌的液体培养及其多糖物质研究[J].菌物学报,1996(1). |
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| 作者姓名： | 朱必凤 马海燕 赵发清 傅明辉 |
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| 作者单位： | 南昌大学生物工程研究所，南昌大学生物工程研究所，南昌大学生物工程研究所，南昌大学生物工程研究所 南昌 330047，南昌 330047，南昌 330047，南昌 330047 |
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| 基金项目： | 中德(江西-0AI)联合研究院基金 |
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| 摘 要： | 采用不同的液体培养基,探索了鸡堆放(土从)菌(Termitomyces albuminosus)人工培养的最适条件,多糖形成的动态变化,并对鸡(土从)多糖进行了分离、纯化,及其免疫活性测定。结果表明:鸡(土从)菌从碳源玉米淀粉,氮源花生蛋白,生长因子麸皮浸汁,pH4.7,25℃为最适生长条件;Sephadex-G100分离多糖出现两个峰;鸡(土从)多糖作为刺激原对人淋巴细胞转化有促进作用。
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| 关 键 词： | 鸡(土从)菌 液体培养 鸡(土从)多糖 淋巴细胞转化 |
STUDIES ON LIQUID CULTURE AND POLYSACCHARIDE OF TERMITOMYCES ALBUMINOSUS |
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| Authors: | Zhu Bifeng Ma Haiyan Zhao Faqing Fu Minghui |
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| Abstract: | Various media were used to study the optimum conditions for the liquid culture of Termitomyces albuminosus and the dynamic variation of forming polysaccharides. The polysaccharides of T'. albuminosus were isolated and purified and their immunological activities were studied. The results show that the optimum conditions for the growth of T. albuminosus are: corn starch as carbon source, peanut protein as nitrogen source, maceration extract of bran as growth factor, pH4.7, temperature 25℃ . Two peaks appeared in the purified polysaccharides by Sephadex G- 100. The polysaccharides of T. albuminosus as stimulant promote the lymphocyte transformation of human. |
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| Keywords: | Termitomyces albuminosus Liquid culture Polysasscaride Lymphocyte transformation |
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