| 随机扩增多态性对白色念珠菌分型的估价 |
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| 引用本文： | 李冬梅,王瑞礼,李若瑜,朱衡,朱立煌.随机扩增多态性对白色念珠菌分型的估价[J].菌物学报,1995,14(2). |
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| 作者姓名： | 李冬梅 王瑞礼 李若瑜 朱衡 朱立煌 |
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| 作者单位： | 北京医科大学真菌和真菌病研究中心 北京 100034；中国科学院遗传所 北京 100101 |
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| 摘 要： | 利用随机扩增多态性(RAPD-PGR)的方法对48株白念珠菌Candida albicans(Robin)Berkh进行了分析。由初步试验中,随机选用了18种引物,筛选出OPA-14引物,该引物扩增的带型清晰可辨,不同菌株之间扩增的带数6—12条不等,共有6条主带。扩增片段的长度粗略估计在300—2000bp左右。除个别菌株的带型相同以外,大多数菌株之间呈多态性分布,其带型的数目和扩增的片段存在差异。对简单的DNA制备方法,以及RAPD-PCR在临床应用和流行病学调查中的可行性进行了初步探讨。
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| 关 键 词： | 白色念珠菌 分型 随机扩增多态性DNA |
ASSESSMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN THE CLASSIFICATION OF CANDIDA ALBICANS |
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| Authors: | Li Dongmei Wang Duanli Li Ruoyu
Research Center of Medical Mycology the First Teaching Hospital Beijing Medical University Beijing
Zhu Heng Zhu Lihuang
Institute of Genetics Academia Sinica Beijing |
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| Institution: | Li Dongmei Wang Duanli Li Ruoyu
Research Center of Medical Mycology,the First Teaching Hospital,Beijing Medical University Beijing 100034
Zhu Heng Zhu Lihuang
Institute of Genetics,Academia Sinica,Beijing 100101 |
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| Abstract: | Randomly amplified polymorphic DNA markers (RAPD-PCR) analysis-was performed in 46 clinic strains and 2 standard strains of Candida albicans. Only the profiles of OPA-14 derived from 18 random primers were very distict. It showed that the amplified numbers of band were from 6 to 12 among different strains. However, there were 6 identical fragments that can be observed in all the amplified profiles of strains, the length of fragments varied from 300 to 2 000bp. Most of strains showed a wide diversity in number and length of amplified fragments. In this paper, the feasibility of application of RAPD-PCR and isolation of fungal DNA by means of benzyl chloride in clinic and epidemiology were also discussed. |
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| Keywords: | Candida albicans Classification Randomly amplified polymorphic DNA markers |
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