鹅膏菌属真菌RAPD分析及亲缘关系的研究

本文对采自湖南莽山的２６种鹅膏菌属（Ａｍａｎｉｔａ）真菌进行了随机扩增多态性ＤＮＡ（ＲＡＰＤ）分析，４０个随机引物中筛选出扩增效果较好的６个引物，每个引物能产生１～１０条ＤＮＡ条带，获得的ＲＡＰＤ谱带清晰并呈现多态性ＯＰＧ１５、ＯＰＨ０４两个引物扩增的ＲＡＰＤ谱带能将２６种鹅膏菌完全区分开来，通过６个引物的ＲＡＰＤ分析获得的平均相似性系数表明种与种之间的相关系数在２０－６０％之间，平均链锁聚类分析可将２６种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。

STUDY ON RELATIONSHIP AMONG SPECIES OFAMANITA USING RAPD

Random amplified polymorphic DNA (RAPD) was used in analysing the relationship amongtwenty-six speices of Amanita from Mangshan,Hunan. of 40 primers, 6 ones generated repreducible RAPDprofiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species ofAmanita by its RAPD profiles. The similarity coefficients obtained from profiles genelated by six primers amongAmanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clusteringanalysis. It indicated that there were tWo groups in 26 species and some species with ring and membrmousvolva clustered a group which coincided with morphological classification.