| 真菌菌丝体培养和提取DNA方法的改进 |
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| 引用本文： | 何月秋.真菌菌丝体培养和提取DNA方法的改进[J].菌物学报,2000,19(3):434. |
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| 作者姓名： | 何月秋 |
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| 作者单位： | 云南农业大学植物病理重点实验室,昆明 650201 |
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| 摘 要： | ANIMPROVEDPROTOCOLFORFUNGALDNAPREPARATIONHEYue-Qiu(KeyLaboratoryofPlantPathology,YunnanAgriculturalUniversity,Kunming650201)DNAfromfUngaJmyceliacanbeinexpensivelypreParedinandcrofugetUbescale.Thesmal1scaleoftheprocedaredescribedheremakesitPOssibleforonepeopletoconvenienUyhandleuPto6osamplesinlessthan5hoursandobtainlO~5OgofDNApersamPle,whichisequaltotheamountenoughtforseveralSouthernhybriclizahonsand5oO~25oOPCRbasedreachons.I.Medium:CaseinhydrolyteO.5g,sucrosel5g,…
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AN IMPROVED PROTOCOL FOR FUNGAL DNA PREPARATION |
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| Authors: | HE Yueqiu |
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| Abstract: | DNA from fungal mycelia can be inexpensively prepared in a microfuge tube scale.The small scale of the procedure described here makes it possible for one people to conveniently handle up to 60 samples in less than 5 hours and obtain l0～50g of DNA per sample,which is equal to the amount enought for several Southem hybridizations and 500～2500 PCR-based reactions. |
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