黑木耳中抑制胰脂肪酶活性物质的提取工艺及体外抑制效果

本文以黑木耳醇提物和水提物为研究对象,以对胰脂肪酶活性的抑制率为指标,分别对其提取工艺进行了单因素和正交试验,选取优化后抑制率高的提取物进行抑制类型和3T3-L1前脂肪细胞方面的研究。结果表明醇提物的最佳提取工艺为提取温度70℃、提取时间1h、乙醇浓度90%、料液比1:20,抑制胰脂肪酶的IC₅₀=681.56μg/mL。水提物的最佳提取工艺为提取温度70℃、提取时间2h、料液比1:40,抑制胰脂肪酶的IC₅₀=850.59μg/mL,醇提物的抑制效果优于水提物。醇提物对胰脂肪酶的抑制类型为非竞争性抑制,抑制常数为4.69mg/mL;醇提物浓度低于1mg/mL时,对3T3-L1前脂肪细胞的活性无影响;浓度高于400μg/mL时即可显著抑制前脂肪细胞的分化。

Extraction technology and inhibitory effects in vitro of substances inhibiting pancreatic lipase activities from fruiting body of Auricaria heimuer

Using inhibition rate of pancreatic lipase activity as index, the extracting process of alcohol extract and water extract of Auricularia heimuer fruiting body (AHFB) were optimized by single factor and orthogonal experiments, respectively. The extract with the highest inhibition rate was used to determine inhibition types and inhibition effects on 3T3-L1 preadipocytes. The results showed that the optimum extracting conditions of ethanol extract of AHFB included the extraction temperature of 70°C, extraction duration of 1h, ethanol concentration of 90%, and solid-liquid ratio of 1:20. IC₅₀ of the ethanol extract inhibiting pancreatic lipase activity obtained was 681.56μg/mL. The optimum extraction conditions of water extract of AHFB were the extraction temperature of 70°C, extraction duration of 2h and solid material-liquid ratio of 1:40. IC₅₀ of the water extract inhibiting pancreatic lipase activity obtained was 850.59μg/mL. The inhibiting rate of ethanol extract on pancreatic lipase activity was higher than that of water extract. The inhibition of ethanol extract on pancreatic lipase was non-competitive, and the inhibition constant was 4.69mg/mL. The viability of 3T3-L1 preadipocytes was not affected when the concentration of ethanol extract was lower than 1mg/mL. The concentration over 400μg/mL could significantly inhibit the differentiation of 3T3-L1 preadipocytes.