SCAR分子标记技术在香菇菌株鉴定上的应用研究

为了建立一套基于DNA分子标记技术快速鉴定香菇菌株的有效方法,本研究首先通过对生产上常用的14个香菇菌株进行RAPD多态性分析,从香菇菌株162中扩增获得了一个片段长为1166bp的特异RAPD标记XG1166,随之利用分子克隆技术将该特异RAPD标记成功转化为稳定的SCAR标记。用同样的方法,本研究又从另一香菇菌株申香10号中获得了一段长度为347bp的特异SCAR标记SX347。试验结果表明,利用本研究获得的香菇菌株162和申香10号的特异SCAR标记,能在一天时间内准确鉴定出香菇菌株162或申香10号菌株的真伪。由此可见,SCAR分子标记是一种快速、稳定、准确鉴定香菇菌株的新方法, 可应用于食用菌种质资源保护利用、品种分类与鉴定和假种辨别。

APPLICATION OF SCAR MOLECULAR MARKER TECHNOLOGY IN IDENTIFICATION OF LENTINULA EDODES

This study was designed for the purpose of establishing an effective way for rapid identification of Lentinula edodes strains based on DNA molecular marker. The RAPD (Random Amplified Polymorphic DNA) analysis was conducted on 14 cultivated strains of Lentinula edodes. A specific RAPD marker with a length of 1166kb, XG1166, could be generated from the Lentinula edodes strain 162. Subsequently, the RAPD marker was converted into more stable SCAR(Sequence Characterized Amplified Region) marker by the use of molecular cloning technology. In the same way, another SCAR marker with a length of 347kb, SX347, was obtained from Lentinula edodes strain Shenxiang-10. The results showed SCAR marker of strain 162 or Shenxiang-10 acquired based on this study could be used as a specific DNA fingerprint to identify strain 162 or Shenxiang-10 whithin one day. Consequently, it was suggested that SCAR molecular marker technology is an effective new way to identify Lentinula edodes strains more accurately and rapidly and it could be applied widely in the protection of germplasm resources, classification and identification distinguishing false strains of edible mushroom.