| 双单杂交技术选育亮盖灵芝与白肉灵芝优良性状杂交子 |
| |
| 引用本文： | 仇昊,田莉,刘笑天,巫涛,葛峰,刘锐,朱静,师亮,于汉寿,赵明文,任昂.双单杂交技术选育亮盖灵芝与白肉灵芝优良性状杂交子[J].菌物学报,2022,41(12):2083-2094. |
| |
| 作者姓名： | 仇昊 田莉 刘笑天 巫涛 葛峰 刘锐 朱静 师亮 于汉寿 赵明文 任昂 |
| |
| 作者单位： | 1 南京农业大学生命科学学院，江苏 南京 2100952 贵州科学院生物研究所，贵州 贵阳 550009 |
| |
| 基金项目： | 国家自然科学基金(81773839);三亚南京农业大学研究院引导资金项目(NAUSY-MS17);贵州省食用菌产业关键领域科技项目(GSGJ004) |
| |
| 摘 要： | 以亮盖灵芝Ganoderma lucidum和白肉灵芝G. leucocontextum为亲本，采用亮盖灵芝异核体双核菌丝与通过原生质体单核化获得的白肉灵芝单核体进行双单杂交，最终获得34株杂交子。采用ISSR技术对34株杂交子及亲本进行DNA多态性聚类分析，杂交子主要分为两个类群。对杂交子进行生长速度和灵芝酸含量对比，筛选到4株优质杂交子。进一步进行杂交子及亲本间HPLC指纹图谱对比，发现4株杂交子中有3株HPLC指纹图谱在具备两个亲本特征灵芝酸的同时，还有低极性灵芝酸的产生。
|
| 关 键 词： | 双单杂交 亮盖灵芝 白肉灵芝 ISSR技术 HPLC指纹图谱 |
| 收稿时间： | 2022-04-06 |
Breeding of Ganoderma lucidum and G. leucocontextum with excellent traits by dikaryon-monokaryon mating |
| |
| Authors: | QIU Hao TIAN Li LIU Xiaotian WU Tao GE Feng LIU Rui ZHU Jing SHI Liang YU Hanshou ZHAO Mingwen REN Ang |
| |
| Institution: | 1 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China2 Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, Guizhou, China |
| |
| Abstract: | Ganoderma lucidum and G. leucocontextum were used as parents for dikaryon- monokaryon mating. Thirty-four hybrids were obtained by dikaryon-monokaryon mating of heterokaryotic Ganoderma lucidum dikaryon mycelium and G. leucocontextum monokaryon mycelium acquired by protoplast mononucleation. ISSR markers were applied for DNA polymorphism cluster analysis of the 34 hybrids and their parents. The hybrids were mainly divided into two groups. Through comparing the growth rate and content of ganoderic acid of all the hybrids, four hybrids with excellent performance in both traits were screened. Comparison of HPLC fingerprints of hybrids and parents found that three of the four hybrids had the characteristics of both parents and there were low-polarity GAs that did not exist in the parents. |
| |
| Keywords: | dikaryon-monokaryon mating Ganoderma lucidum G leucocontextum ISSR method HPLC fingerprint |
|
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
