黑柄炭角菌(乌灵菌)粉改善睡眠作用以及对小鼠大脑神经递质及其受体的影响

本研究探讨了黑柄炭角菌菌核乌灵菌粉的改善睡眠作用以及对小鼠大脑神经递质及其受体的影响。144例小鼠随机分为低、中、高3个剂量(0.25、0.5和1.0 g/kg bw)的乌灵菌粉实验组和1个空白对照组,进行了30 d的灌胃干预,从小鼠睡眠行为和其大脑组织中神经递质及其受体两个方面进行分析。结果显示,乌灵菌粉无直接催眠作用,对小鼠正常生长和体重增长无显著影响。相比于对照组,乌灵菌粉3个剂量实验组巴比妥钠诱导的入睡潜伏期均显著缩短(P<0.05),戊巴比妥钠阈下剂量下入睡动物数均有增加,戊巴比妥钠诱导的睡眠时间仅有中、高两个剂量组显著增加(P<0.05),低剂量组睡眠时间增加不显著(P>0.05)。低、中、高3个剂量组小鼠大脑组织中γ-氨基丁酸A型受体(GABA_AR)表达量均显著上升(P<0.05),γ-氨基丁酸B型受体(GABA_BR)表达量却显著下降(P<0.05),5-羟色胺(5-HT)和γ-氨基丁酸含量均显著上升(P<0.01)。综上所述,乌灵菌粉具有明显的改善睡眠功效,其作用机制可能是通过提高脑中5-HT和GABA含量,促进小鼠大脑组织中GABA_AR受体表达来实现。

Xylaria nigripes (wuling) sclerotium powder improving sleep and influencing the expression of brain neurotransmitters and their receptor of mice

Sleeping improvement effects of Xylaria nigripes sclerotium powder on mice and the effect of the power on neurotransmitters and their receptor in brain of mice were investigated. A total of 144 mice was divided randomly into three experimental groups (treated with the dosages of 0.25, 0.5, 1.0 g/kg bw) and one control group, and the gavage lasted 30 d. Sleep behavior and the expression of neurotransmitter receptors in brain of mice were analyzed. The results showed that, the experimental samples had no direct hypnotic effect and might not affect the growth of body weight. Compared with control group, the sleep latency time induced by barbital sodium decreased (P<0.05) and the sleeping rate increased under the threshold hypnogenesis dosage of pentobarbital sodium in three treatment groups. Sleep time in 0.25 g/kg bw treatment group has no significant increase (P>0.05) while 0.5 and 1.0 g/kg bw treatment group significantly extended sleep time under sodium pentobarbital induction (P<0.05). After administration of Xylaria nigripes sclerotium powder, the content of GABA_A receptor (GABA_AR) (P<0.05), 5-hydroxytryptamine (5-HT) (P<0.01) and GABA (P<0.01) in the brain of mice was found to be augmented while that of GABA_B receptor (GABA_BR) was decrease (P<0.05). In conclusion, Xylaria nigripes sclerotium powder could increase the content of GABA_AR, 5-HT and GABA in the brain and thus achieve sleeping improvement effect.