林下不同菌种皱环球盖菇产量与环境因子相关性分析

以林下种植的不同菌种皱环球盖菇Stropharia rugosoannulata为研究对象,利用高通量测序、相关分析、回归分析和方差分析等方法,分析产量与气象环境因子以及土壤环境因子的相关性。相关性分析显示：日降雨量、空气湿度、土壤水分和大气压对皱环球盖菇各菌种日产量有关键响应,高产菌种S.ra1和S.ra9与日降雨量呈一次函数模型;9个皱环球盖菇菌种平均日产量与5个属细菌相对丰度以及土壤速效钾含量呈显著相关,与blank_CK样地比较,高产菌种S.ra9提高芽单胞菌属Gemmatimonas细菌相对丰度,从而提高土壤固氮作用,高产菌种S.ra1提高芽单胞菌属Gemmatimonas、粘液杆菌属Mucilaginibacter和固定杆菌属Conexibacter细菌相对丰度,从而提高土壤固氮作用、降解栽培基质纤维素作用以及土壤可溶性有机碳含量,说明皱环球盖菇高产菌种S.ra1和S.ra9的栽培可提高林地土壤肥力。

The correlation of yield of various Stropharia rugosoannulata cultivated strains with forest environmental factors

The correlations of yield of various Stropharia rugosoannulata cultivated strains with forest environmental factors, such as meteorological factors and soil factors, were investigated by using the combination of Illumina MiSeq sequencing platform, correlation analysis, regression analysis and variance analysis. It showed that daily rainfall, atmospheric humidity, soil moisture and atmospheric pressure were the key response factors to daily yield of different S. rugosoannulata strains. The daily yield of the strains S.ra1 and S.ra9 had a linear relationship with daily rainfall. The correlations of average daily yield of nine S. rugosoannulata strains with soil environmental factors, such as available potassium and abundance of five bacterial genera, were significant. Compared with the blank_CK plot, the abundance of soil-inhabitan Gemmatimonas in S.ra9 cultivated plot significantly increased, as a result, the soil nitrogen fixation in the plot improved. Compared with the blank_CK plot, the abundance of soil bacterial genera Gemmatimonas, Mucilaginibacter and Conexibacter in the S.ra1 cultivated plot significantly increased, resulting in the increase of soil nitrogen fixation, degradation ability of cellulose and content of dissolved organic carbon. In summary, cultivation of high yielding strains S.ra1 and S.ra9 under forest environment would effectively improve soil fertility of forest land.