黑曲霉木聚糖酶的纯化与性质 |
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引用本文: | 陈红歌,朱静,梁改芹,严自正,贾新成,张树政.黑曲霉木聚糖酶的纯化与性质[J].菌物学报,2000,19(1). |
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作者姓名: | 陈红歌 朱静 梁改芹 严自正 贾新成 张树政 |
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作者单位: | 中国科学院微生物研究所 |
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基金项目: | 国家自然科学基金!39600029,国家“九五”科技攻关项目!No.96-C03-02-03 |
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摘 要: | 由凝胶电泳酶谱检测到黑曲霉149发酵液中存在两型木聚糖酶,依次为X-Ⅰ和X-Ⅱ.通过硫酸铵分级沉淀及DEAE-SephadexA50柱层析分别将X-Ⅰ、X-Ⅱ纯化到凝胶电泳均一。由SDS一凝胶电泳和浓度梯度凝胶电泳测得X-Ⅰ和X-Ⅱ的分子量分别为37kDa,76kDa,24kDa和23kDa,X-Ⅰ具有亚基。二者的含糖量分别为276%和7.3%。X-Ⅰ和X-Ⅱ最适反应温度分别为50℃和55℃,pH为46和5.2。在pH4.6~9.2和pH4.0~10.0之间X-Ⅰ、X-Ⅱ活力稳定。50℃保温24h,X-Ⅰ活力仍为100%,而X-Ⅱ的活力已降为2.8%。HgCl2和AgNO3显著抑制X-Ⅰ、X-Ⅱ的活力。X-Ⅰ与X-Ⅱ水解不同来源的木聚糖,其产物有所不同。
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关 键 词: | 黑曲霉 木聚糖酶 纯化 性质 |
PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER |
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Authors: | CHEN Hong-Ge ZHU Jing LIANG Gai-QinYAN Zi-Zheng JIA Xin-Cheng ZHANG Shu-Zheng |
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Abstract: | Zymograms of the cultrual supernatant of Aspergillus niger 149 showed xylanase's two bandstentatively named X-I and X-II. X-I and X-II were purified to PAGE homogenity by (NH4)2So4 fractionationand DEAE-Sephadex A50 column chlomatography. The molecular mass of X-I and X-II eshmated by SDS-PAGEand concentrahon gradient PAGE were 37kDa,76kDa,24kDa, and 23kDa. respechvely. X-I and X-II contained27.6% and 7.3% carbohydrate residues, respechvely. The optimal reachon conditions for X-I and X-II were at50℃ pH4.6 and 55℃ PHS.2. X-I and X-II retained 100% and 2.8% respechvely, of the original activity afterheating at 50℃ for 24h. The achvihes of X-I and X-II were considerably inhibited by HgCl2 and AgNO3. Thehydrolysis products from different xylan with X-I and X-II were different. |
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Keywords: | mpergillus niger Xylanase Purification Propertis |
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