| 一种快速高效提取病原真菌DNA作为PCR模板的方法 |
| |
| 引用本文： | 陈吉良,黄小龙,吴安迪,周双清,黄东益,刘进平.一种快速高效提取病原真菌DNA作为PCR模板的方法[J].菌物学报,2011,30(1):147-149. |
| |
| 作者姓名： | 陈吉良 黄小龙 吴安迪 周双清 黄东益 刘进平 |
| |
| 作者单位： | 海南大学农学院,儋州,571737 |
| |
| 基金项目： | 高等学校博士学科点专项科研基金（No. 200805650004）；农业部热带作物种质资源专项（农垦办[2009]58） |
| |
| 摘 要： | 真菌rDNA-ITS序列分析适合于较高等级水平的生物群体间的系统分析。真菌DNA的提取采用传统的方法，步骤繁琐，需要较长时间。采用Chelex-100法提取真菌DNA，使用PCR扩增rDNA-ITS序列评价提取核酸的质量。结果显示，该方法具有经济、简便、快速、高效的特点，是一种比较理想的提取真菌基因组DNA作为PCR模板的方法。
|
| 关 键 词： | 真菌DNA Chelex-100法 DNA提取 |
An efficient extraction method of pathogenic fungus DNA for PCR |
| |
| Authors: | CHEN Ji-Liang HUANG Xiao-Long WU An-Di ZHOU Shuang-Qing HUANG Dong-Yi and LIU Jin-Ping |
| |
| Institution: | Agronomy College, Hainan University, Danzhou 571737, China;Agronomy College, Hainan University, Danzhou 571737, China;Agronomy College, Hainan University, Danzhou 571737, China;Agronomy College, Hainan University, Danzhou 571737, China;Agronomy College, Hainan University, Danzhou 571737, China;Agronomy College, Hainan University, Danzhou 571737, China |
| |
| Abstract: | The fungal rDNA-ITS sequence analysis is suitable for systematic studies of higher level biological populations. However, this traditional method of extraction of fungal genomic DNA is procedurally overelaborate and time-consuming. Chelex-100 was adopted to extract DNA from pathogenic fungi, and the qualities of PCR amplification and rDNA-ITS sequence analysis were evaluated. The results showed that this improved method is simple, high efficient and convenient for pathogenic fungal DNA extraction. |
| |
| Keywords: | fungal DNA Chelex-100 method DNA extraction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
|
