小麦条锈菌条中31号生理小种SCAR检测标记的建立

建立小麦条锈菌Pucciniastriiformisf.sp.tritici生理小种的快速分子检测技术对我国小麦条锈病的监测和防治策略的制定具有重要价值,本文首次报道了利用SCAR—PCR技术进行条锈菌生理小种分子检测的方法。通过对我国目前主要优势小种条中31号RAPD片段的规模筛选,在对特异片段回收、克隆、测序的基础上,设计特异PCR引物,成功获得了条中31号生理小种专化的SCAR检测标记。

THE DEVELOPMENT OF SCAR DETECTION MARKER OF PUCCINIA STRIIFORMIS F. SP. TRITICI RACE CY31 IN CHINA

Rapid identification of the races of Puccinia striiformis f. sp. tritici by molecular technique was very important to monitor and control of wheat stripe rust in China. In this paper, a molecular identification method for a Chinese race of Puccinia striiformis f. sp. tritici CY31 was developed by means of SCAR-PCR technique. Five Chinese races of P. striiformis f. sp. tritici were analysed with 210 primers by RAPD technique. A special DNA fragment of race CY31 was founded and cloned. Based on the sequencing result, specific PCR primers were designed and a SCAR marker for race CY31 was obtained successfully. The results here indicates that the identification of the races of Puccinia striiformis f. sp. tritici could be improved greatly through cosmically searching special RAPD fragments of different races.