量天尺根部内生真菌的多样性

为了解不同量天尺（火龙果）品种根部内生真菌菌群组成及多样性,采集GHL-1、GHL-2、GHL-3、ML-1和DL 5个量天尺品种健康根部样品,进行内生真菌分离,采用形态观察和ITS序列分析相结合的方法进行鉴定、归类。共分离得到内生真菌菌株117株,总体分离率为25.71%,分别隶属于13个属,其中Trichoderma、Fusarium、Chaetomium和Phoma为量天尺内生真菌的优势种群,分别占总菌株数的24.79%、35.04%、10.26%和10.26%;不同量天尺品种内生真菌的结构和组成存在一定差异,GHL-2、GHL-3和DL 3个品种中分离频率最高的内生真菌类群为Fusarium,GHL-1和ML-1分离频率最高的类群为Trichoderma;多样性分析结果反映出不同量天尺品种内生真菌菌群的多样性指数、丰富度指数和均匀度指数水平存在差异,其中GHL-2的3项指数均为最高。表明品种差异对内生真菌的组成和多样性均有影响。

Diversity analysis of endophytic fungi from Hylocereus undatus

The endophytic fungi isolated from the roots of five cultivated varieties of Hylocereus undatus (GHL-1, GHL-2, GHL-3, ML-1 and DL) were classified based on morphological observation and ITS sequence analysis. A total of 117 endophytic fungal isolates was obtained with average isolating frequency of 25.71%, belonging to 13 genera of which Trichoderma (24.79%), Fusarium (35.04%), Chaetomium (10.26%) and Phoma (10.26%) were dominant. Fungal population structure and specific composition of endophytic fungi varied with different varieties. The highest isolation frequency of Fusarium was obtained in GHL-2, GHL-3 and DL, and that of Trichoderma was obtained in GHL-1 and ML-1. The diversity index, richness index and evenness index of endophytic fungi varied with different varieties, and GHL-2 showed the highest. The results indicated that the diversity and specific composition of endophytic fungi were obviously affected by disparity in varieties.