| 一种无孢灵芝菌丝体的活性成分 |
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| 引用本文： | 岳亚文,周帅,冯杰,王金艳,唐庆九,刘艳芳,张劲松,冯娜,韩伟.一种无孢灵芝菌丝体的活性成分[J].菌物学报,2020,39(1):128-136. |
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| 作者姓名： | 岳亚文 周帅 冯杰 王金艳 唐庆九 刘艳芳 张劲松 冯娜 韩伟 |
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| 作者单位： | 1 华东理工大学药学院制药工程与过程化学教育部工程研究中心 上海市新药设计重点实验室 上海 2002372 上海市农业科学院食用菌研究所 国家食用菌工程技术研究中心 农业部南方食用菌资源利用重点实验室 上海市农业遗传育种重点开放实验室 上海 201403 |
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| 摘 要： | 利用制备型高效液相、凝胶层析、制备型薄层色谱等方法对无孢灵芝龙芝2号的固体发酵菌丝体进行分离纯化,通过波谱分析,化合物分别鉴定为灵芝酸P（1）,灵芝酸T1（2）,灵芝酸Mk（3）,灵芝酸S（4）,灵芝酸T（5）,ganodermanondiol（6）,灵芝酸Me（7）,5α, 8α-epidioxyergosta-6, 22-dien-3β-ol（8）,灵芝酸R（9）,lanosta-7, 9(11), 24-trien-3α-hydroxy-26-oic acid（10）,ganodermenonol（11）。其中灵芝酸T1为首次发现的天然产物。体外细胞实验证实,11种化合物对肿瘤细胞L1210的增殖均有很强的抑制作用,其增殖抑制的IC50值均在39.69μmol/L以下。
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| 关 键 词： | 固体发酵 三萜 甾醇 生物活性 |
| 收稿时间： | 2019-05-23 |
Active components in mycelia of sporeless Ganoderma lingzhi |
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| Authors: | YUE Ya-Wen ZHOU Shuai FENG Jie WANG Jin-Yan TANG Qing-Jiu LIU Yan-Fang ZHANG Jing-Song FENG Na HAN Wei |
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| Institution: | (Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China) |
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| Abstract: | Eleven compounds were isolated from mycelial solid culture of sporeless Ganoderma lingzhi Longzhi No. 2 by preparative high-performance liquid chromatography(HPLC), gel chromatography and preparative thin layer chromatography(PTLC), etc. On the basis of their spectral data, they were identified as ganoderic acid P(1), ganoderic acid T1(2), ganoderic acid Mk(3), ganoderic acid S(4), ganoderic acid T(5), ganodermanondiol(6), ganoderic acid Me(7), 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol(8), ganoderic acid R(9), lanosta-7, 9(11), 24-trien-3α-hydroxy-26-oic acid(10), and ganodermenonol(11). Ganoderic acid T1 was first discovered as natural product. All the compounds showed strong proliferation inhibition to tumor cell L1210 with IC50 values of <39.69μmol/L. |
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| Keywords: | solid-state fermentation triterpenoids steroids bioactivity |
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