高氧条件下灵芝子实体活性氧含量及其代谢相关酶活性

灵芝Ganoderma lingzhi是最著名的药用真菌之一。本文研究了60%高氧条件下灵芝子实体呼吸速率、灵芝酸（ganoderic acid,GA）含量、总酚含量、活性氧（reactive oxygen species,ROS）含量、丙二醛（malondialdehyde,MDA）含量、抗氧化酶活性、黄嘌呤氧化酶（xanthineoxidase,XOD）活性、琥珀酸脱氢酶（succinic dehydrogenase,SDH）活性、H ⁺-ATP酶活性、Ca ²⁺-ATP酶活性的变化。结果显示,高氧抑制灵芝子实体的呼吸速率;处理前期（第1天）,灵芝子实体内过氧化氢（H₂O₂）和超氧阴离子自由基（O₂ ^-?）含量高于对照组,但随着处理的进行,ROS含量显著减少,MDA积累减少,超氧化物歧化酶（superoxide dismutase,SOD）、过氧化氢酶（catalase,CAT）和SDH活性提高,GA和总酚含量增加。表明一定的环境胁迫压力可以激发灵芝启动自身的抗氧化系统,保护机体免受氧化损伤,并促进相关次生代谢产物的合成。

Changes of reactive oxygen species content and related metabolic enzyme activities in the fruiting body of Ganoderma lingzhi under high oxygen condition

The respiratory rate,ganoderic acid(GA)content,total phenolic content,reactive oxygen species(ROS)content,malondialdehyde(MDA)content,antioxidant enzyme activities,xanthineoxidase(XOD)activity,succinic dehydrogenase(SDH)activity,H^+-ATPase and Ca^2+-ATPase in Ganoderma lingzhi fruiting body were investigated under the treatment of 60%oxygen.The results showed that high oxygen inhibited the respiratory rate of G.lingzhi fruiting body,and the content of H2 O2 and O2^-·were higher than that in experimental control in the first day.However,the production of ROS retarded with the development of high oxygen treatment,and the MDA content reduced.The activities of SOD,CAT and SDH were enhanced and the content of GA and total phenol increased.It is suggested that suitable environmental stress can stimulate the fungus to activate its antioxidant system to protect itself from oxidative damage,and enhance the biosynthesis of related secondary metabolites.