三株亚肉座菌乙酸乙酯提取物生物活性及GC-MS分析

采用乙酸乙酯提取3株亚肉座菌菌丝体,测试虫生真菌乙酸乙酯提取物（EAE）的抗肿瘤、抗菌和抗氧化活性,并借助GC-MS方法分析各提取物中的化学成分。结果发现2株亚肉座菌的菌丝体EAE对HepG2细胞的抑制活性较强,IC₅₀均小于9μmol/L;抗菌结果表明2株真菌的提取物具有抑制细菌生长的作用;1株供试菌的EAE表现出较强的DPPH自由基清除活性（清除率可达85%）。GC-MS分析表明从亚肉座菌JXJG201717、JXJG201720和ARSEF7697的EAE中分别鉴定出21、35和39种成分,主要成分为酯类、醇类和酸类;盘状亚肉座菌JXJG201720与ARSEF7697有相同化合物13个,与暹罗亚肉座菌JXJG201717存在7个相同化合物。本研究表明虫生亚肉座菌具有产生丰富活性成分的能力,彰显出多种利用价值。

Bioactivities and GC-MS analyses of ethyl acetate extracts from three strains of Hypocrella (Clavicipitaceae,Ascomycota)

Mycelium ethyl acetate extracts (EAE) of three Hypocrella strains were used to detect their antitumor, antibacterial and antioxidant activities, and to analyze their chemical compositions by GC-MS. The results showed that EAE of two Hypocrella strains exhibited strong inhibitory activities against HepG2 cell, with an IC₅₀ value of less than 9μmol/L. Antibacterial test results indicated that EAE of two Hypocrella strains displayed inhibiting activities against Escherichia coli, Bacillus subtilis and Xanthomonas pruni. One tested Hypocrella strain possessed higher DPPH radical scavenging activities (scavenging rate up to 85%). GC-MS analyses showed that twenty-one, thirty-five and thirty-nine kinds of constituents were obtained from EAE of entomogenous Hypocrella JXJG201717, JXJG201720 and ARSEF7697, respectively, which were mainly esters, alcohols and acids. Both H. discoidea JXJG201720 and ARSEF7697 had thirteen same compounds. JXJG201720 and JXJG201717 shared seven same compounds. The present study unveiled that entomogenous Hypocrella was possessed of various bioactive components with antitumor, antibacterial and antioxidant properties, with multiple values of utilization.