灵芝菌丝体中一个三萜类化合物的核磁归属及活性初探

通过液体振荡-静置两阶段发酵获得灵芝菌丝体,并采用硅胶柱色谱层析、反相柱层析和甲醇重结晶的方法,从中分离得到4个三萜类化合物。根据NMR、MS等波谱数据分析,化合物分别被鉴定为lanosta-7,9(11),24-trien-3α-acetoxy-26-oic acid（1）、灵芝酸R（2）、灵芝酸T（3）和灵芝酸S（4）,其中化合物1的核磁信号全归属为首次报道。4个三萜类化合物均具有较好的抑制肿瘤细胞L1210及K562增殖的活性,且化合物1的体外抗肿瘤活性为首次证实,其对肿瘤细胞L1210及K562增殖的半数抑制浓度IC₅₀分别为22.17μmol/L和54.79μmol/L。

NMR attribution and bioactivity evaluation of a triterpene in mycelia of Ganoderma lingzhi

Ganoderma lingzhi mycelia were obtained by liquid shaking-static two-stage fermentation method, and four triterpenes were isolated from the mycelia through silica gel column chromatography, reversed-phase column chromatography and methanol recrystallization. According to the analysis of spectral data of NMR and MS, these compounds were identified as lanosta-7,9(11),24-trien-3α-acetoxy-26-oic acid (1), ganoderic acid R (2), ganoderic acid T (3) and ganoderic acid S (4). The nuclear magnetic signals of compound 1 were reported for the first time. All of the four triterpenes showed good inhibitory activity on the proliferation of tumor cells L1210 and K562, and the in vitro antitumor activity of compound 1 was confirmed for the first time. IC₅₀ values of compound 1 against tumor cells L1210 and K562 were 22.17μmol/L and 54.79μmol/L, respectively.