荧光素钠和考马斯亮蓝应用于小麦白粉病菌染色效果比较*

比较了荧光素钠和考马斯亮蓝应用于小麦白粉病菌染色的效果。荧光素钠法中样品处理只需20min.左右,具有直接、快速的特点;荧光指示剂对病菌分生孢子萌发及菌丝生长无抑制作用,主要沉集于活菌体的隔膜和细胞质部位,使病菌产生明显的亮绿荧光和清晰的细胞轮廓,亮绿荧光衰退期为7min.;借助荧光显微镜可以观察病菌在小麦叶表的发展过程,区别活菌体和失活菌体。考马斯亮蓝法包括传统的组织学染色步骤,经过改进后的样品处理过程需要40min.左右;染色后使寄主组织呈现淡蓝色,病菌菌体染成深蓝色;该方法可以观察病菌在小麦叶表和被侵染细胞内部发育形成的结构,包括孢子发育形成的初生芽管、附着胞芽管、成熟附着胞以及在寄主细胞内形成的初生吸器原体、成熟的指状体吸器和次生吸器。

COMPARISON OF STAINING EFFECTS OF URANINE AND COOMASSIE BRILLIANT BLUE R-250 ON BLUMERIA GRAMINIS F.SP. TRITICI

Staining effects of uranine and Coomassie brilliant blue R-250 on Blumeria graminis f.sp. tritici was observed. The results showed that uranine staining need only 20 min. of sample treatment, without inhibiting pathogen growth. Uranine was mainly accumulated in the septum and cytoplasm of living pathogen, maintaining brilliant green fluorescence for about 7 min. By use of fluorescence microscopy, the development of the pathogen on the wheat leaves surface could be detected and the dead and alive pathogen could be distinguished. Coomassie brilliant blue R-250 took about 40 min. for treating sample, and the host cell was turned into light blue, and pathogen dark blue. This is a rapid staining method for observing infection structures of B. graminis f.sp. tritici on wheat epidermis or in infected cell, such as primary germtube, appressorial germtube, mature appressoria, pristine haustorium and secondary finger-like haustoria as well as conidia.