| 草菇聚酮合酶编码基因vv-alb的克隆及其表达的初步分析 |
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| 引用本文： | 林楠,茅文俊,汪虹,冯爱萍,鲍大鹏.草菇聚酮合酶编码基因vv-alb的克隆及其表达的初步分析[J].菌物学报,2012,31(1):142-149. |
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| 作者姓名： | 林楠 茅文俊 汪虹 冯爱萍 鲍大鹏 |
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| 作者单位： | 国家食用菌工程技术研究中心农业部应用真菌资源与利用重点开放实验室 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海201403 |
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| 基金项目： | 上海市农业科学院青年基金[农青年科技2009（09）] |
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| 摘 要： | 聚酮化合物(polyketides)是一类庞大的次级代谢家族,聚酮合酶(polyketide synthase,PKS)是介导聚酮化合物生物合成的关键酶。通过巢氏简并PCR与染色体步行的方法,获得了草菇中的编码PKS的基因vv-alb的全长序列,并通过荧光实时定量RT-PCR方法对vv-alb基因在草菇不同生长阶段与不同部位的表达情况进行了初步分析,为进一步研究PKS在草菇和其他食用真菌生物代谢过程中的作用奠定了一定的基础。
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| 关 键 词： | 草菇 聚酮合酶 荧光实时定量RT-PCR |
Cloning and expression analysis of a polyketide synthase gene vv-alb from the edible straw mushroom, Volvariella volvacea |
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| Authors: | LIN Nan MAO Wen-Jun WANG Hong FENG Ai-Ping and BAO Da-Peng |
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| Institution: | National Research Center for Biotechnology and Engineering of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi,;National Research Center for Biotechnology and Engineering of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi,;National Research Center for Biotechnology and Engineering of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi,;National Research Center for Biotechnology and Engineering of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi,;National Research Center for Biotechnology and Engineering of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, |
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| Abstract: | Polyketides are secondary metabolites that exhibit a broad range of biological activities. The key enzyme involved in biosynthesis is polyketide synthase (PKS). We report the cloning of a putative PKS gene (vv-alb) by nested PCR using degenerate primers and chromosome walking from the edible straw mushroom, Volvariella volvacea. The gene vv-alb is 6,704bp in size and contains a 6,279bp coding region (encoding 2,093 amino acid residues) and eight introns. The encoded product of the vv-alb gene contains four functional domains: ketosynthase (KS), acyl transferase (AT), thioesterase (TE) and acyl carrier protein (ACP), the typical characteristics of PKS. Real-time quantitative PCR analysis revealed that vv-alb expression was 5-10-fold higher in the stipe region as compared with the vegetative mycelium, primordia and pileus. |
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| Keywords: | Volvariella volvacea polyketide synthase real-time quantitative PCR |
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