飞虱虫疠霉分生孢子在不同温湿度组合条件下的存活率* |
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引用本文: | 徐均焕,冯明光.飞虱虫疠霉分生孢子在不同温湿度组合条件下的存活率*[J].菌物学报,2001,20(1):68-72. |
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作者姓名: | 徐均焕 冯明光 |
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作者单位: | 浙江大学微生物研究所 |
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基金项目: | 国家自然科学基金!(39870513,30070025),国家科技部!(G73项目),浙江省教委基金 |
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摘 要: | 用荧光染色法研究了不同温度(10~30℃)和湿度(51~100%RH)组合条件下飞虱虫疠 霉 Pandora delphacis分生孢子的存活率。在无放回抽样观察中,孢子弹射后第24 h的存活 率在51%、74%、85%、90%、95%和100%RH与不同温度的组合中分别为42~81%、69~89%、70~ 95%、 67~100%、 76~100%和 56~100%。存放4个月后,不同温度下的孢子存活率在 51%、 74%、 85%和 90%RH下分别为 55~74%、 52~87%、 38~73%和 1~65%。而在≥95% RH下孢子存活率 24h 后锐减,至第7 d几乎全部失活。以上结果表明,弹射后的飞虱虫疠霉孢子在头24 h的存活 易受低湿影响,但存活下来的孢子能继续在低湿下存活较久;饱和或接近饱和的高湿度最不 利孢子长久存活。
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关 键 词: | 飞虱虫疠霉 孢子存活率 环境因子 |
文章编号: | 1007-3515(2001)01-0073-0077 |
修稿时间: | 2000年7月19日 |
IMPACT OF TEMPERATURE AND RELATIVE HUMIDITY ON PERSISTENCE OF PRIMARY CONIDIA OF THE ENTOMOPHTHORACEOUS FUNGS, PANDORA DELPHACIS |
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Authors: | XU Jun-Huan FENG Ming-Guang |
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Abstract: | A fluorescence microscopy on a basis of fluorescein diacetate and propidium iodide was used to investigate the persistence of primary conidia of Pandora delphacis (Entomophthorales) at combinated regimes of temperature (10~30℃) and relative humidity (51~100% RH). Examination of the conidia on glass coverslips sampled 24 h after discharge resulted in a survival rate of 42~81% at 51% RH, 69~89% at 74% RH, 70~95% at 85% RH, 67~100% at 90% RH, 76~100% at 95% RH, and 56~100% at 100% RH, respectively, varying with different temperature. Maintained at 10~30℃ for 4 mon, the rate of survial conidia remained 55~74% at 51%RH, 52~87% at 74% RH, 38~73% at 85% RH, and 1~65% at 90% RH, respectively. However, 7 d after discharge, few conidia were found surviving at 95% RH regardless of the temperature. The results indicate that the first 24 h after discharge was crucial to longer survival of P. delphacis conidia at the humidity of 74% RH whereas saturated or nearly saturated air was not favorable to their longer survival. |
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Keywords: | Pandora delphacis Conidia persistence Environmental temperature and relative humidity |
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