| 黄曲霉毒素B_1抗独特型抗体的制备和应用研究II.抗独特型抗体应用 |
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| 引用本文： | 陈福生,周启,罗信昌,李书谦,王爱华,路磊.黄曲霉毒素B_1抗独特型抗体的制备和应用研究II.抗独特型抗体应用[J].菌物学报,2004(2). |
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| 作者姓名： | 陈福生 周启 罗信昌 李书谦 王爱华 路磊 |
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| 作者单位： | 教育部农业微生物重点实验室农业部食品安全评价重点开放实验室 武汉430070
(陈福生，周启，罗信昌)，农业部食品质量监督检验测试中心 武汉430064
(李书谦，王爱华)，农业部食品质量监督检验测试中心 武汉430064(路磊) |
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| 基金项目： | 湖北省科技厅重点课题(992p0321)，教育部农业微生物重点实验室开放课题(AML99023) |
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| 摘 要： | 以抗独特型抗体(anti-idiotypeantibody,Ab2)的酶切片段Fab2替代黄曲霉毒素B1(AFB1)建立一种不需要使用AFB1的无毒酶联免疫吸附(Enzyme-LinkedImmunosorbentAssay,ELISA)试剂盒,研究该试剂盒特异性、稳定性和AFB1的加标回收,并将该试剂盒用于农产品和饲料中AFB1的检测。结果表明,该试剂盒具有和常规ELISA试剂盒一样的特异性和加标回收能力,无毒试剂盒和常规试剂盒一样都可以用于农产品和饲料中AFB1的检测,并且两种试剂盒的检测结果并无显著差异,无毒ELISA试剂盒在适当处理后,40C放置3个月和-200C放置5个月,以间接非竞争ELISA测定的吸光值分别是起始时的85%和87%。
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| 关 键 词： | ELISA试剂盒 食品安全 农产品 |
PREPARATION AND APPLICATION OF AFLATOXIN B_1(AFB_1) ANTI-IDIOTYPE ANTIBODY II. APPLICATION OF ANTI-AFB_1IDIOTYPE ANTIBODY |
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| Authors: | CHEN Fu-Sheng ZHOU Qi LUO Xin-Chang |
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| Abstract: | Non-toxin ELISA was developed with fragment antigen-binding(Fab2) of anti-idiotype antibody (Ab2)substituting aflatoxin B1(AFB1). The specificity, AFB1 recovery in spiked samples and shelf-life of the non-toxin ELISA kit were investigated, and the kit was used in detecting AFB1 from agricultural commodities and feed. The results revealed that the non-toxin ELISA kit had the same specificity and AFB1 recovery ability with the regular ELISA kit, and both ELISAs could be used in detecting AFB1 from agricultural commodities and feed. The non-toxin ELISA kits were stored in 40C for three months and -200C for 5 months after appropriate treatment, the OD490nm values of kits in indirect ELISA were 85% and 87% of original OD490nm values, respectively. |
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| Keywords: | ELISA Kit food safety agricultural commodities |
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