| 蛇足石杉内生真菌无柄盘菌Pezicula sp. JR14中抑制乙酰胆碱酯酶的次级代谢产物分离 |
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| 引用本文： | 郑瑞,张志斌,颜日明,汪涯,肖依文,朱笃.蛇足石杉内生真菌无柄盘菌Pezicula sp. JR14中抑制乙酰胆碱酯酶的次级代谢产物分离[J].菌物学报,2018,37(1):102-109. |
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| 作者姓名： | 郑瑞 张志斌 颜日明 汪涯 肖依文 朱笃 |
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| 作者单位： | 1 江西师范大学 江西省亚热带植物资源保护与利用重点实验室 江西 南昌 3300222 江西科技师范大学生命科学学院 江西省生物加工过程重点实验室 江西 南昌 330013 |
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| 基金项目： | 国家自然科学基金（81760649,31760160）;江西省自然科学基金（20171BAB204009）;江西省教育厅科技项目（GJJ161624） |
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| 摘 要： | 采用形态学及ITS-rDNA序列分析法,对具有抑制乙酰胆碱酯酶活性的蛇足石杉内生菌株JR14进行鉴定,确定为无柄盘菌Pezicula sp.。开展了乙酰胆碱酯酶抑制活性化合物的分离,从Pezicula sp. JR14乙酸乙酯提取物中分离到4个化合物,利用核磁共振及质谱技术将其鉴定为behenic acid (1)、himeic acid B (2)、secalonic acid A (3)和palmitic acid (4)。采用改进的Ellman比色法对分离的化合物进行活性检测,结果表明,himeic acid B (2)具有较强的乙酰胆碱酯酶抑制活性,其IC50为205μg/mL（0.64mmol/L）。
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| 关 键 词： | 蛇足石杉 内生真菌 无柄盘菌 乙酰胆碱酯酶抑制剂 |
| 收稿时间： | 2017-10-29 |
Isolation of secondary metabolites with acetylcholinesterase inhibitory activity of endophytic fungus Pezicula sp. JR14 from Huperzia serrata |
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| Authors: | Rui ZHENG Zhi-Bin ZHANG Ri-Ming YAN Ya WANG Yi-Wen XIAO Du ZHU |
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| Institution: | 1 Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, Jiangxi 330022, China2 Key Lab of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi 330013, China |
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| Abstract: | An endophytic fungus strain JR14 with acetylcholinesterase (AChE) inhibitory activity from Huperzia serrata was identified as Pezicula sp. by morphological characteristics and internal transcribed spacer (ITS) sequence analysis. Four kinds of secondary metabolites were separated and purified from the ethyl acetate extract of the endophytic Pezicula sp. JR14. According to mass spectrometry, 1H-NMR and 13C-NMR analyses, they were identified as behenic acid (1), himeic acid B (2), secalonic acid A (3) and palmitic acid (4). Ellman colorimetric test proved that himeic acid B (2) showed better AChE inhibitory activity with IC50 value of 205μg/mL (0.64mmol/L). |
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| Keywords: | Huperzia serrata endophytic fungus Pezicula sp acetylcholinesterase inhibitor |
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