| 细脚拟青霉菌丝体多糖纯化及组成分析 |
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| 引用本文： | 刘桂君,文华安.细脚拟青霉菌丝体多糖纯化及组成分析[J].菌物学报,2008,27(6):947-955. |
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| 作者姓名： | 刘桂君 文华安 |
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| 作者单位： | 1. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101;北京顺鑫农业股份有限公司牛栏山酒厂,北京,101301
2. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101 |
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| 摘 要： | 通过DE-52纤维素柱和Sephedex G-100葡聚糖凝胶柱,对细脚拟青霉Paecilomyces tenuipes菌丝体粗多糖进行纯化,得到PtPs1和PtPs2,应用离子色谱HPAEC-PAD对纯多糖PtPs1和PtPs2的单糖组成进行分析,证实均由葡萄糖、半乳糖和甘露糖三种单糖组成,经红外扫描确定PtPs1和PtPs2均为α型吡喃糖。
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| 关 键 词： | 纤维素柱 葡聚糖凝胶柱 红外光谱 |
Purification and component analysis of polysaccharides from mycelia of Paecilomyces tenuipes |
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| Authors: | LIU Gui-Jun and WEN Hua-An |
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| Institution: | Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Niulanshan Distillery, Beijing Shunxin Agriculture Limited Company, Beijing 101301, China;Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The polysaccharides of Paecilomyces tenuipes mycelia were purified by using DEAE cellulose DE-52 and Sephedex G-100, and pure polysaccharides PtPs1 and PtPs2 were obtained. The structural unit (monosaccharide) of PtPs1 and PtPs2 was analyzed by HPAEC-PAD. The results indicated that both PtPs1 and PtPs2 consisted of glucose, galactose and mannose. The infrared spectral analysis showed that PtPs1 and PtPs2 were all pyranose of α-configuration. |
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| Keywords: | DEAE-cellulose chromatogram sephadex chromatogram infrared spectrum |
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