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| 桦褐孔菌液体深层培养研究 | |
| 引用本文： | 王振河,霍云凤,王斌,申进文.桦褐孔菌液体深层培养研究[J].菌物学报,2006,25(3):461-467. |
| 作者姓名： | 王振河 霍云凤 王斌 申进文 |
| 作者单位： | 1. 河南科技学院,新乡,453003 2. 河南科技学院,新乡,453003;贵州大学发酵工程与生物制药贵州省重点实验室,贵阳,550003 3. 河南省科学院生物研究所,郑州,450008 |
| 摘 要： | 研究了不同碳源、氮源、生长因子和无机盐对桦褐孔菌液体深层培养菌丝体产量的影响。采用“二次回归正交组合设计”对培养基进行优化,结果表明,当培养基配方为小麦淀粉水解糖100.83g/L(还原糖含量为24%)、酵母粉18.70g/L、玉米浆11.01g/L、KH2PO44.59g/L、MgSO4·7H2O1.5g/L、pH6.0,在30℃、160r/min的恒温培养振荡器上振荡培养120h,生物量可达19.80g/L。 |
| 关 键 词： | 药用蕈菌 营养因子 二次回归正交组合设计 生物量 |
| 文章编号： | 1672-6472（2006）03-0461-0467 |
| 收稿时间： | 2005-09-15 |
| 修稿时间： | 2006-03-13 |
Submerged fermentation of Phaeoporus obliquus |
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| Authors: | WANG Zhen-He HUO Yun-Feng WANG Bin SHEN Jin-Wen |
| Abstract: | The effects of different carbon resources, nitrogen sources, growth factors and inorganic salts on biomass of Phaeoporus obliquus were studied. Optimum medium was obtained by using duality quadratic regress composite intersect design. The result showed that the maximal biomass was 19.80g/L when P. obliquus strains grew in 500mL flasks containing hydrolysate of amylose (100.83g/L, DE 24%),powdery yeast (18.70 g/L),corn steep liquor(11.01 g/L),KH2PO4( 4.59 g/L),and MgSO4·7H2O (1.5g/L),on a rotary shaker under the condition of 160r/min for 120h at 30℃. |
| Keywords: | Medicinal mushroom nutrition factors duality quadratic regress composite intersect design biomass |
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