| 运用mRNA差异显示技术研究黄伞发育相关基因 |
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| 引用本文： | 季哲,李玉祥,赵明文,潘迎捷.运用mRNA差异显示技术研究黄伞发育相关基因[J].菌物学报,2007,26(2):243-248. |
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| 作者姓名： | 季哲 李玉祥 赵明文 潘迎捷 |
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| 作者单位： | 1. 南京农业大学,农业部农业环境微生物工程重点开放实验室,南京,210095
2. 上海水产大学,上海,200090 |
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| 摘 要： | 采用mRNA差异显示技术对黄伞菌丝体、原基、菇蕾、子实体菌柄和子实体菌盖进行研究,获得了在生殖生长阶段差异表达的4条cDNA片段T_(11)G B0304、T_(11)G B0304-2、T_(11)G B0322与T_(11)C B0310,经过回收、重扩增、克隆与鉴定后进行测序分析,结果发现T_(11)G B0322的序列在GenBank中与氧类固醇结合蛋白(oxysterol-binding protein)基因序列的同源性达到41％。再经过半定量PCR方法验证,表明T_(11)G B0322基因片段是在生殖生长阶段差异表达的基因片段。
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| 关 键 词： | 差异cDNA片段 氧类固醇结合蛋白 半定量PCR |
| 文章编号： | 1672-6472（2007）02-0243-0248 |
| 收稿时间： | 7/1/2006 12:00:00 AM |
| 修稿时间： | 2006-07-012006-07-29 |
Study on differential development gene of Pholiota adiposa bv DDRT-PCR |
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| Authors: | JI Zhe LI Yu-Xiang ZHAO Ming-Wen and PAN Ying-Jie |
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| Institution: | Key Lab of Microbiological Engineering of Agricutural Environment of Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiological Engineering of Agricutural Environment of Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiological Engineering of Agricutural Environment of Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Shanghai Fisheries University; Shanghai 200090 |
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| Abstract: | RNA of different development periods of pholiota adiposa including mycelium,anlage,bud and fruit body(stem and pileus of mushroom)was extracted and treated by RT-PCR.Four fragments(T_(11)G B0304,T_(11)G B0304-2,T_(11)G B0322 and T_(11)C B0310)were isolated,reamplified,purified,cloned and sequenced.After blasting in GenBank,T_(11)G B0322 cDNA fragment was identified to be similar to oxysterol-binding protein.The result of semiquantitive PCR showed that the expression of the T_(11)G B0322 cDNA fragment was different in different periods.This showed that gene fragment might be associated with differentiation of Ph.adiposa. |
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| Keywords: | different cDNA fragment oxysterol-binding protein semiquantitive PCR |
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