| 用灭活原生质体融合进行高温香菇育种——Ⅰ.融合产物的选出及其鉴定 |
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| 引用本文： | 彭卫宪,陆大京.用灭活原生质体融合进行高温香菇育种——Ⅰ.融合产物的选出及其鉴定[J].菌物学报,1987(3). |
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| 作者姓名： | 彭卫宪 陆大京 |
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| 作者单位： | 华南师范大学生物系 广州
(彭卫宪)，广东省微生物研究所 广州(陆大京) |
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| 摘 要： | 用灭活的近裸香菇(Lentinus subnudus Berk.)双核菌株原生质体与香菇L. edodes(Berk.)Sing.]双核菌株原生质体融合,在35℃的条件下选得融合子。融合频率为0—4.3×10~(-5)。融合子与双亲有明显的拮抗性。融合子的菌丝形态、氨基酸含量,子实体的形态,以及酸性磷酸酶同功酶的测定都与双亲不同。
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| 关 键 词： | 近裸香菇 香菇 灭活原生质体 融合 |
STUDIES ON THE BREEDING OF MUSHROOMS BY MEANS OF INACTIVATED PROTOPLAST FUSION——I. STUDIES ON THE SELECTION AND DETERMINATION OF FUSION PRODUCTS |
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| Authors: | PENG WEI-XIAN |
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| Abstract: | Fusion strains have been obtained by means of high temperature (35℃) selection after fusion between the inactivated protoplasts of Lentinus subnudus Berk. dicaryons and living protoplasts of L. edodes (Berk.) Sing. dicaryons. Fusion frequencies survived after protoplast fusion were 0-4.3×10~(-5). There are obvious antagonism among fusion strains and their parents. Morphology and aminoacid content of hyphae, and fruiting bodies morphology of fusion strains are different from those of their parents. |
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| Keywords: | Lentinus subnudus L edodes Inactivated protoplast Fusion |
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