从天然蛹虫草不同部位分离菌株特性比较

以采集自泰山地区的6株野生蛹虫草为研究对象,分别通过菌核、子座组织分离和子囊孢子分离法共获得18个菌株,经ITS鉴定均为蛹虫草菌株后,进一步对18个菌株进行了菌丝生长速度、菌落形态、出草试验、主要活性物质（虫草素、腺苷、虫草多糖、β-类胡萝卜素、叶黄素）和交配型的测定分析。结果表明：在菌丝长势、出草产量和整齐度方面,从子囊孢子分离得到的菌株普遍优于组织分离菌株;而在活性物质含量方面,从子囊孢子和子座分离得到的菌株普遍优于菌核分离菌株。通过交配型测定发现,从菌核、子座和子囊孢子分离得到的18个菌株均不存在交配型缺失现象。

Variation of biological characteristics of Cordyceps militaris strains isolated from different parts of the natural samples

Sclerotia, stromata and ascospores of six wild samples of Cordyceps militaris collected from Mount Tai were applied to isolate the fungus strains. The variations of mycelial growth rate, colony morphology, cultivation characteristics, active components (cordycepin, adenosine, polysaccharide, β-carotenoid, lutein) and mating-type genes of 18 strains obtained were measured. The results showed that mycelial growth, fruiting body yield and fruiting uniformity of the strains isolated from ascospores were superior; the content of active components of the strains isolated from ascospores and stromata were much higher than that from sclerotia. All tested strains contained both MAT-1 and MAT-2 mating type genes.