蝉棒束孢子实体的致畸性评价及对高血糖小鼠血糖的影响

对妊娠第7-16天Wistar大鼠连续给予口服蝉棒束孢子实体低、中、高3个剂量（0.85、1.70、3.40g/kg）10d,观察给药组对大鼠孕期体重、死胎率、吸收胎率、活胎数、每窝平均胎仔数、胎鼠重、胎鼠身长、前囟宽度、子宫连胎重及胎鼠外观、内脏发育、骨骼发育的影响,研究妊娠动物接触蝉棒束孢子实体后引起的致畸可能性。对正常小鼠、STZ致高血糖模型小鼠给予口服不同剂量的人工培植蝉棒束孢子实体（0.4、0.25、0.125g/kg）,测定空腹血糖及糖耐量,评价蝉棒束孢子实体的降血糖作用。结果表明：与阴性对照组相比,人工培植蝉棒束孢子实体各剂量组在大鼠孕期体重、死胎率、吸收胎率、胎鼠重、胎鼠身长和前囟宽度、胎鼠外观、内脏和骨骼发育等方面均无显著性差异（P>0.05）;与模型对照组（0g/kg BW）相比,人工培植蝉棒束孢子实体中、低两个剂量组均能降低STZ致高血糖模型小鼠给葡萄糖后0.5h血糖水平（P<0.05）,低剂量组能明显降低高血糖模型小鼠0-2h血糖曲线下面积,且对小鼠体重及正常小鼠空腹血糖均无不良影响。提示人工培植蝉棒束孢子实体对大鼠无明显的母体毒性、胚胎毒性和致畸性;对高血糖小鼠具有降血糖的作用。

Effects of administration of Isaria cicadae fruiting bodies on fetal mouse teratogenicity and blood sugar of hyperglycemic mice

Artificially cultivated Isaria cicadae fruiting bodies (ACICF) (with dosages of 0.85, 1.70, 3.40g/kg) were continuously orally administered for 10 days to Wistar rats on the 7th to 16th day of pregnancy. The effects on pregnancy body weight, stillbirth rate, absorbed fetal rate, number of live fetuses, average number of fetuses per litter, fetal weight, fetal length, bregma width, uterine including fetal weight, fetal appearance, internal organ development, and fetal bone development were investigated to study the possibility of teratogenicity of pregnant animals contacting ACICF. Meanwhile, different doses of ACICF (0.4, 0.25, 0.125g/kg) were orally administered to normal mice and STZ-induced hyperglycemia model mice, and fasting blood glucose and glucose tolerance were measured to evaluate hypoglycemic effect of ACICF. The results showed that each ACICF-treated group had no significant difference as compared with the negative control group in the weight, stillbirth rate, absorbed fetal rate, fetal mouse weight, fetal mouse body length and bregma width, fetal mouse appearance, and development of internal organ and bone (P>0.05). Compared with the model control group (0g/kg BW), both the medium and low dose ACICF-treated groups could reduce the blood glucose level of STZ-induced hyperglycemia model mice in 0.5h after administration of glucose (P<0.05), and the low dose treatment could significantly reduce the area under the 0-2h blood glucose curve of hyperglycemia model mice. ACICF had no adverse effects on the weight of the mice and the fasting blood glucose of normal mice. It is suggested that ACICF has no obvious toxicity and teratogenicity to maternal rats and embryo and it has a hypoglycemic effect on hyperglycemic mice.