牛樟芝提取物调控Nrf2/HO-1通路对酒精性肝损伤的保护作用

研究不同剂量（100、200和400mg/kg）的牛樟芝水提物（WE）、醇提后水提取物（WEE）和醇提物（EE）对酒精诱导的ICR小鼠急性肝损伤的保护作用和对Nrf2/HO-1抗氧化信号通路的影响。研究结果表明：与模型组比较,400mg/kg的WE和WEE均能显著抑制血清ALT和AST水平的升高,200mg/kg的WE和WEE分别显著降低血清ALT和AST含量。各剂量的WE、WEE和EE均能显著降低肝脏MDA含量,200和400mg/kg的WE和不同剂量的WEE均可明显提高肝脏的SOD和CAT活力。H&E染色结果表明WE、WEE和EE对酒精诱导的肝损伤均有一定的改善作用,EE处理组的效果相对较差。免疫组化染色结果表明各剂量的WE、WEE和EE均能促进Nrf2的核转位,诱导HO-1的表达,提高肝脏的抗氧化能力,对酒精诱导的急性肝损伤具有明显的保护作用。提示牛樟芝能通过调节Nrf2/HO-1抗氧化信号通路发挥解酒保肝功效。

Hepatoprotective effects of extracts of Taiwanofungus camphoratus on liver with alcohol-induced injury through regulation of Nrf2/HO-1 signaling pathway

Protective effects of different doses (100, 200 and 400mg/kg) of water extract (WE), water extract after extraction by ethanol (WEE) and ethanol extract (EE) of Taiwanofungus camphoratu on alcohol-induced acute liver injury in ICR mice, as well as Nrf2/HO-1 antioxidant signaling pathway were investigated. Compared with the model group, 400mg/kg dosage of both WE and WEE could inhibit the increase of serum ALT and AST levels significantly, and 200mg/kg of WE and WEE could obviously decrease the serum ALT and AST content, respectively. Different doses of WE, WEE and EE could significantly reduce the content of hepatic MDA; 200 and 400mg/kg of WE and different doses of WEE could markedly increase hepatic SOD and CAT activities. H&E staining observation indicated that WE, WEE and EE could mitigate alcoholic liver injury to a certain degree, but a relative lower effect was observed in EE-treated groups. The immunohistochemical staining results indicated that different dose of WE, WEE and EE could enhance the nuclear translocation of Nrf2 and promote the expression of HO-1 to improve the hepatic antioxidant capacity, thus presenting a significant protective effect on alcohol-induced acute liver injury. It was suggested that Taiwanofungus camphoratus exhibited drunkenness-alleviating and liver-protecting effects via regulating the Nrf2 antioxidant signaling pathway.