蓝光和蛋白酶体抑制剂MG132对蛹虫草形态的影响

蓝光是影响生物生长发育过程的重要因素,同时生物个体的生长发育过程中不断有蛋白质的泛素化降解。采用蓝光和蛋白酶体抑制剂MG132处理蛹虫草菌,观察蛹虫草菌落、菌丝体和子实体形态的变化。研究结果表明,黑暗条件下正常生长的菌落边缘圆滑一致,菌落之间融合无边界;MG132处理后,菌落之间出现明显的界限,边缘菌丝稀疏。蓝光条件下无MG132处理时,菌落较为单薄,转色明显;MG132处理时,菌落中间橙色,边缘颜色变淡。扫描电镜观察,黑暗条件下无MG132处理的菌丝自然弯曲,菌丝表面较为光滑,菌丝粗细差别不大且分枝较少,分生孢子表面较光滑。黑暗条件下MG132处理菌丝体,菌丝较直且分枝较多,菌丝容易断裂,分生孢子表面仍较光滑。蓝光条件下菌丝体弯曲减少,菌丝表面较为粗糙,单条菌丝常出现部分区段膨大呈不规则状,菌丝粗细差异较大,菌丝断裂较多;分生孢子呈扁平的椭圆状,表面有纹理,且粗糙。蓝光条件下MG132处理的菌丝体,菌丝较直,表面粗糙,菌丝整体变得更细,菌丝断裂较多;分生孢子呈不规则形状,表面纹理更深,粗糙有褶皱。此外,MG132可导致蛹虫草子实体畸形生长。结果表明,蓝光和MG132均可以影响蛹虫草的形态变化。

Morphological observation of Cordyceps militaris treated with blue light and proteasome inhibitor MG132

Blue light is an important factor in the growth and development of organisms, and ubiquitination degradation of proteins are constantly occurring during the growth and development of organisms. Cordyceps militaris was treated with blue light and proteasome inhibitor MG132 to observe the changes of colony, mycelium and fruiting body morphology. The results showed that the edges of normal colonies growing under dark condition were smooth and consistent, and there was no boundary between colonies. When the fungus was treated with MG132, obvious boundaries appeared between colonies and marginal hyphae were sparse. Under the blue light condition without MG132 treatment, the colony is relatively thin and the color change is obvious. When the fungus is treated with MG132, colonies are orange in the center and pale at the edges. Scanning electron microscope observation showed that the mycelia without MG132 treatment were naturally curved under dark condition, with smooth surface, and uniform thickness and few branches, and the surface of conidia was smooth. Under dark condition, the mycelia treated with MG132 were comparatively straight and more branched, breakable, and the surface of conidia was still smooth. Scanning electron microscope observation indicated that under the blue light condition the mycelium curvature decreased, and the mycelium surface was rough; the mycelium often partially and irregularly expanded and differed greatly in thickness, and easily fractured; the conidia were flattened, ellipsoid, veined, and coarse in surface. Under the blue light condition, the mycelia treated with MG132 were comparatively straight, superficially rough, as a whole became thinner, and easily broken; the conidia were irregularly shaped, superficially deep-veined, rough and wrinkled. In addition, MG132 can cause abnormal growth of fruiting body of C. militaris. These results indicated that both blue light and MG132 could affect the morphological changes of C. militaris.