中国被毛孢发酵液的活性成分分析及清除自由基和抗白色假丝酵母活性的研究

测定处于不同生长期的中国被毛孢Hirsutella sinensis菌株RCEF0273胞外多糖含量、发酵液中5种核苷代谢量和氨基酸含量,并对其菌株次生代谢产物清除自由基活性和抑菌效果进行了测定分析,发现:中国被毛孢胞外多糖含量最高可达到1.9480g/L,发酵液中尿苷、鸟苷、肌苷、胸腺嘧啶核苷和腺苷5种核苷含量最高分别为10.4132mg/g、12.1929mg/g、2.2698mg/g、1.1000mg/g和1.8181mg/g,其菌株发酵液冻干粉中氨基酸总量为3.2560mg/g,活性测定结果显示中国被毛孢发酵液的正丁醇相有很强的清除自由基活性和抑菌活性。在10mg/mL的测试浓度下,37℃孵育15min后其自由基清除率可达93.25%,在2mg/mL的测试浓度下,其抗白色假丝酵母Candida albicans的抑菌圈直径达到21.42mm。

Some active components, free radical scavenging activity and anti-Candida albicans activity of the fermentation broth of Hirsutella sinensis

The extracellular polysaccharide and nucleosides of the fermentation broth of Hirsutella sinensis RCEF0273 at different growth stages and the amino acids of the freeze-dried fermentation broth were analyzed. Free radical scavenging activity and anti-Candida albicans activity of the extracts of the fermentation broth of RCEF0273 were also detected. The results suggested that the highest contents of the extracellular polysaccharide,uridine,guanosine,inosine,thyminide and adenosine of the fermentation broth reached 1.9480g/L,10.4132mg/g,12.1929mg/g,2.2698mg/g,1.1000mg/g and 1.8181mg/g respectively. The content of amino acids of the freeze-dried fermentation broth reached 3.2560mg/g. Bioactive tests showed that n-butanol extract of the fermentation broth had the strongest free radical scavenging activity and inhibition activity. At the concentration of 10mg/mL,and incubation at 37℃ for 15min,the free radical scavenging activity reached 93.25%. At the concentration of 2mg/mL,the n-butanol extract had a 21.64mm inhibition ring.