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| 双孢蘑菇基质降解能力退化的差异蛋白质组学分析 | |
| 引用本文： | 陈美元,王泽生,廖剑华,李洪荣,蔡志欣,谢宝贵.双孢蘑菇基质降解能力退化的差异蛋白质组学分析[J].菌物学报,2011,30(3):508-513. |
| 作者姓名： | 陈美元 王泽生 廖剑华 李洪荣 蔡志欣 谢宝贵 |
| 作者单位： | 1. 福建省农业科学院食用菌研究所,福州,350014;福建农林大学茵物研究中心,福州,350002 2. 福建省农业科学院食用菌研究所,福州,350014 3. 福建农林大学茵物研究中心,福州,350002 |
| 基金项目： | 国家自然科学基金（No. 30871769），福建省重大专题（No. 2008SZ0002） |
| 摘 要： | 对双孢蘑菇CGMCC No.0214及其基质降解能力退化菌株0214-3、0214-5进行了蛋白质双向电泳(2-DE或2D-PAGE)分析,发现了2个明显的、可重复的差异蛋白质,在退化菌株中分别为上调和下调表达,且2个退化菌株表现一致。根据质谱(MALDI-TOF/MS)分析和数据库检索结果,2个差异蛋白质初步鉴定为肌动蛋白和NADH脱氢酶铁硫蛋白3。 |
| 关 键 词： | 食用菌 蛋白质组 双向电泳 差异蛋白质 质谱分析 |
Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus |
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| Authors: | CHEN Mei-Yuan WANG Ze-Sheng LIAO Jian-Hu LI Hong-Rong CAI Zhi-Xin and XIE Bao-Gui |
| Institution: | Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China Mycological Research Center of Fujian Agriculture and Forestry University, Fuzhou 350002, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Mycological Research Center of Fujian Agriculture and Forestry University, Fuzhou 350002, China |
| Abstract: | The technique of two-dimensional electrophoresis (2-DE or 2D-PAGE) was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No. 0214 and its substrate-decomposing ability degenerated strains 0214-3, 0214-5. As a result, two proteins were found to be up- and down-expressed, respectively, with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively. The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A. bisporus. |
| Keywords: | edible fungus proteome two-dimensional electrophoresis differential protein MALDI-TOF/MS analysis |
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