农杆菌介导的橡胶草遗传转化体系的建立

以橡胶草为试验材料，利用农杆菌介导法进行遗传转化研究。结果表明：50 mg/L的卡那霉素(Kan)对橡胶草的抗性芽具有很好地筛选效果，生根筛选时，Kan的适宜质量浓度为35 mg/L；最佳抑菌抗生素为羧苄青霉素（cb），适宜质量浓度为500 mg/L，获得35株抗性试管苗，通过GUS染色及分子生物学检测，最终获得6株转基因植株，转化率为17.1%。可见，获得的橡胶草转基因植株，为橡胶草后续的遗传转化研究奠定了基础。

Establishment of Agrobacterium-mediated Genetic Transformation System for Taraxacum kok-saghyz

Using the Taraxacum kok-saghyz Rodin as experimental material, we proceeded the Agrobacterium-mediated genetic transformation research. The results showed that 50 mg/L Kan had a very good screening effect to the resistance buds of Taraxacum kok-saghyz, in rooting screening research, the appropriate mass concentration of Kan was 35 mg/L ;the best antibacterial antibiotics was carbenicillin (cb) with the mass concentration of 500 mg/L . 35 resistant test-tube plantlets were obtained, after screening of GUS staining and molecular biological detection, 6 transgenic plants were determined finally, the transformation rate was 17.1%. The established system set the foundation for the genetic transformation of Taraxacum kok-saghyz.