九种蟋蟀mtDNA-16S rRNA基因序列及其系统进化(直翅目:蟋蟀科)

蟋蟀科5属9个种的线粒体16S rRNA基因部分序列被测定或从Gen Bank获得，比较其同源性，计算核苷酸使用频率，并构建NJ和MP分子系统树。在获得的449bp的序列中A、T、C和G碱基含量分别为31．8％、36．9％、9．9％和21．4％，A T平均含量为68．7％。研究结果表明：所研究的5属9种蟋蟀聚成3个聚类簇，斗蟋属先与灶蟋属汇合，再与棺头蟋属构成聚类簇Ⅰ；油葫芦属黑脸油葫芦和北京油葫芦与蟋蟀属的家蟋相聚构成聚类簇Ⅱ；蟋蟀属的田蟋单独构成聚类簇Ⅲ。

Phylogenetic Relationships of Nine Crickets Based on Partial Sequences of 16S rRNA Gene(Orthoptera:Gryllidae)

The partial sequences of mitochondrial 16S rRNA gene (449bp) were sequenced for 9 species of crickets belonging to 5 genera of Gryllidae. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular trees reconstructed by Neighbor-Joining method and maximum parsimony method using Dianemobius taprobanensis as outgroup. In the obtained sequences of crickets, the content of A, T, C and G was 31.8%, 36.9%, 9.9% and 21.4% respectively, and A% + T% was 68.7%. The reseach results suggested that 9 individuals from the 5 genera clustered in 3 clusters, among which genus Velarifictorus and Gryllodes firstly clustered and then together with genus Loxoblemmus to form cluster I; Cluster II was made of three species, Teleogryllus emma, T. mitratus and Gryllus domesticus; G. campestris single as cluster III.