| Abstract: | Reconstituted high density lipoprotein has been prepared by sonication and preparative ultracentrifugation of mixtures containing the apoprotein of high density lipoprotein, egg phosphatidylcholine, cholesteryl oleate, and acyl chain deuterated cholesteryl palmitate in aqueous buffer. The resulting structures have a size and chemical composition very similar to native high density lipoprotein. Deuterium NMR spectra and longitudinal relaxation times were obtained at approximately 25 degrees C. The variation of the 2H NMR line width with chain position is consistent with an average conformation such that the ester acyl chain is extended. In addition, 2H NMR line widths and longitudinal relaxation times indicate that the ester acyl chains possess significant mobility. |
