Dynamin is functionally coupled to insulin granule exocytosis |
| |
Authors: | Min Le Leung Yuk M Tomas Alejandra Watson Robert T Gaisano Herbert Y Halban Philippe A Pessin Jeffrey E Hou June Chunqiu |
| |
Institution: | Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York 11794, USA. |
| |
Abstract: | The insulin granule integral membrane protein marker phogrin-green fluorescent protein was co-localized with insulin in Min6B1 beta-cell secretory granules but did not undergo plasma membrane translocation following glucose stimulation. Surprisingly, although expression of a dominant-interfering dynamin mutant (Dyn/K44A) inhibited transferrin receptor endocytosis, it had no effect on phogringreen fluorescent protein localization in the basal or secretagogue-stimulated state. By contrast, co-expression of Dyn/K44A with human growth hormone as an insulin secretory marker resulted in a marked inhibition of human growth hormone release by glucose, KCl, and a combination of multiple secretagogues. Moreover, serial pulse depolarization stimulated an increase in cell surface capacitance that was also blocked in cells expressing Dyn/K44A. Similarly, small interference RNA-mediated knockdown of dynamin resulted in marked inhibition of glucose-stimulated insulin secretion. Together, these data suggest the presence of a selective kiss and run mechanism of insulin release. Moreover, these data indicate a coupling between endocytosis and exocytosis in the regulation of beta-cell insulin secretion. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|