Angiotensin I-converting enzyme inhibitory peptides in red-mold rice made by Monascus purpureus |
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| Authors: | Megumi Kuba Kumi Tanaka Masayasu Sesoko Fumihide Inoue Masaaki Yasuda |
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| Institution: | 1. Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan;2. Department of Health Sciences, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan;3. Laboratory of Food Development, Ryukyu Cement Co., Ltd., 162 Yamanoha, Nago-city, Okinawa 905-0008, Japan;4. Department of medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan |
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| Abstract: | The ACE inhibitory activity in red-mold rice extracts, prepared from 24 strains of the genus Monascus, was measured. The most effective strain for ACE inhibition was Monascus purpureus IFO 4489 (IC50 = 0.71 mg/ml). Although the antihypertensive substance γ-amino butyric acid was detected in the red-mold rice (85.2 mg/kg), it did not contribute to ACE inhibition. Four ACE inhibitory peptides were isolated from the extract and identified as Ile-Tyr (IC50 = 4.0 μM), Val-Val-Tyr (22.0 μM), Val-Phe (49.7 μM) and Val-Trp (3.1 μM) by protein sequencing. The ACE inhibitory activity of these peptides was almost completely preserved after successive in vitro digestion by pepsin, chymotrypsin and trypsin. These results suggest that red-mold rice made by M. purpureus could be useful in alleviating hypertension. |
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| Keywords: | Red-mold rice Monascus purpureus Angiotensin I-converting enzyme inhibitor Hypertension Bioactive peptide Tofuyo |
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