Potential of Aqueous Two-Phase Systems constructed on flexible devices: Human serum albumin as proof of concept |
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| Institution: | 1. CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;2. CESAM – Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;3. Instituto Politécnico de Coimbra, ESTESC-Coimbra Health School, Complementary Sciences, 3046-854 Coimbra, Portugal;1. Chemistry Department, CICECO, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;2. São Paulo University, Av. Prof. Lineu Prestes n 580 Bloco 16, 05508-000 São Paulo, SP, Brazil;1. Centro de Química Estrutural, Instituto Superior Tecnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal;2. Instituto de Tecnologia Química e Biologica Antonio Xavier, Universidade Nova de Lisboa, Av. da Republica, 2780-157 Oeiras, Portugal;1. Hospital Universitario 12 Octubre, Madrid, Spain;2. Hospital Universitario Marques De Valdecilla, Santander, Spain;3. Urology Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY;4. Department of Urology, Weill Medical College of Cornell University, New York, NY |
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| Abstract: | In the present research, the potential use of flexible disposable devices, specifically blood bags, for the fractionation of biological products using Aqueous Two-Phase Systems (ATPS) polymer–salt is studied and demonstrated. Purified human serum albumin (HSA) was used as model protein. Experiments were carried out on ATPS polyethylene glycol (PEG)–potassium phosphate constructed on rigid recipients (conical tubes) and flexible devices (blood bags). The device used for ATPS construction had no significant effect on HSA partition behavior. Protein partition towards the top phase was favored on systems constructed using PEG 1000 g/mol and TLL 45% (w/w), achieving up to 85% recovery. On the other hand a recovery of 92% was achieved at the bottom phase when PEG 3350 g/mol and TLL 25% (w/w) were used. Human serum was used as a complex sample on ATPS experiments. Selective fractionation of human serum proteins on ATPS constructed on flexible devices was achieved. ATPS constructed on blood bags required short equilibrium times (< 6 min), meaning it is feasible to use this approach on mass scale. The potential use of flexible disposable devices, for the fractionation of biological products using ATPS polymer–salt was demonstrated. |
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