Purification and molecular docking study of angiotensin I-converting enzyme (ACE) inhibitory peptides from hydrolysates of marine sponge Stylotella aurantium |
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| Institution: | 1. Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;2. Department of Food Science, Faculty of Agricultural Technology, University of Brawijaya, Malang 65145, Indonesia;3. Department of BioMolecular Sciences and Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA;4. Vaccine Research and Development Center, National Health Research Institutes, Miaoli 35053, Taiwan;5. Research Center for Tropic Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;6. Research Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;1. Department of Biological Sciences, University of Limerick, Limerick, Ireland;2. Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada |
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| Abstract: | Angioteinsin I-converting enzyme (ACE) inhibitory peptide was isolated from marine sponge (Stylotella aurantium) hydrolysate prepared by various hydrolysis enzymes. The peptic hydrolysate exhibited highest ACE inhibitory activity among them and was fractionated into three ranges of molecular weight. The below 5 kDa fraction showed the highest ACE inhibitory activity and was used for subsequent purification steps. The amino acid sequences of the purified peptides were identified to be Tyr-Arg (337.2 Da), and Ile-Arg (287.2 Da). The purified peptides from marine sponge had an IC50 value of 237.2 μM and 306.4 μM, respectively. The molecular docking study revealed that ACE inhibitory activity of the purified peptides was mainly attributed to the hydrogen bond interactions and Pi interaction between the dipeptides and ACE. The results suggest that marine sponge, S. aurantium would be an attractive raw material for the manufacture of anti-hypertensive nutraceutical ingredients. |
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| Keywords: | Marine sponge Angioteinsin I-converting enzyme (ACE) Molecular docking Peptides |
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