Anti-oxidative effects of silkworm storage protein 1 in HeLa cell |
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| Institution: | 1. Department of Genetic Biochemistry, Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland;2. Department of Physico-Chemistry of Microorganisms, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland;3. Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Economics and Engineering, University of Economics, Komandorska 118/120, 50-345 Wroc?aw, Poland;4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Number 348, Xian Jia Hu Xi Road, Yue Lu District, 400205 Changsha City, PR China;5. Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, pl. Grunwaldzki 24A, 50-363 Wroclaw, Poland;6. Linum Foundation, Pl. Grunwaldzki 24a, 50-363 Wroclaw, Poland;1. DONG Energy, Kraftværksvej 53, DK-7000 Fredericia, Denmark;2. Center for BioProcess Engineering, Dept. of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark;1. Department of Biotechnology, Dr B R A National Institute of Technology Jalandhar, 144011, Punjab, India;2. Department of Chemistry, Dr B R A National Institute of Technology Jalandhar, 144011, Punjab, India;1. Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, 2050, South Africa;2. Faculty of Chemistry, Opole University, Pl. Kopernika 11, 45-040 Opole, Poland;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China;4. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;1. Protein Structure Group, Korea Basic Science Institute, Ochang, Chungbuk 28119, South Korea;2. College of Pharmacy, Korea University, Sejong 30019, South Korea;3. Department of Biotechnology, Korea University Graduate School, Seoul 02841, South Korea;4. Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon 34133, South Korea;5. Bioimaging Research Team, Korea Basic Science Institute, Ochang, Chungbuk 28119, South Korea;6. Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34133, South Korea;7. Center for Convergent Research of Emerging Virus Infection(CEVI), Korea Research Institute of Chemical Technology, Daejeon 34114, South Korea;8. Department of Microbiology, Kyungpook National University School of Medicine, Deagu 41944, South Korea |
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| Abstract: | Silkworm hemolymph contains unique proteins that exhibit anti-apoptotic activity in mammalian cells. Among them, 30 K protein, which is one of the major anti-apoptotic molecules in silkworm hemolymph, has been well investigated. However, little is known about the biological functions of storage protein 1 (SP1), another main protein in silkworm hemolymph. In this study, the anti-apoptotic and anti-oxidative activities of SP1 were analyzed. A stable cell line expressing SP1 was constructed, which showed strong anti-apoptotic effect induced by staurosporine treatment. In addition, the cell line exhibited resistance to oxidative stress caused by hydrogen peroxide. For practical applications of SP1, recombinant SP1 was produced in Escherichia coli, and the supplementation of recombinant SP1 into culture medium exhibited anti-apoptotic and anti-oxidative activities. In addition, SP1 was found to be a cell-penetrating protein and localized in the cytosol as well as on the plasma membrane. The findings showed that SP1 itself is not an anti-oxidant; rather, it mediates intracellular anti-oxidative activity. In conclusion, the cellular resistance of SP1 to apoptosis and oxidative stress will provide a new strategy that could be utilized in the bio-industry for the production of biologics as well as for the development of anti-aging cosmetics. |
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| Keywords: | Anti-apoptotic activity Anti-oxidative activity Oxidative stress Silkworm Storage protein 1 |
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