Development of Novel Glucose oxidase Immobilization on Graphene/Gold nanoparticles/Poly Neutral red modified electrode |
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| Institution: | 1. Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran;2. Mechanical Engineering Department, Islamic Azad University, Roudehen branch, Roudehen, Tehran, Iran;1. Department of Biotechnology, Tula State University, Pr. Lenina 92, Tula 300012, Russia;2. Department of Chemistry, Tula State University, Pr. Lenina 92, Tula 300012, Russia;3. Laboratory of Cytology of Microorganisms, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pr. Nauki 5, Pushchino, Moscow Region 142290, Russia;4. Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia;1. Department of Immunology, State Research Institute Center for Innovative Medicine, Zygimantu 9, LT-01102, Vilnius, Lithuania;2. Department of Materials Science and Electronics, Institute of Semiconductor Physics, State Scientific Research Institute Center for Physical Sciences and Technology, A. Gostauto 11, LT-01108, Vilnius, Lithuania;3. Department of Analytical and Environmental Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania;4. Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania;5. Institute of Microbiology, National Academy of Sciences, Belarus, Kuprevich 2, BL-220141, Minsk, Belarus;1. Graduate School of Energy and Environment, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea;2. School of Chemical Engineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea;1. School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA;2. Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea;1. Department of Biomedical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA, United States;2. Center for Polymer-Based Protein Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA, United States;3. Department of Materials Science & Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA, United States;4. Disruptive Health Technology Institute, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA, United States;5. Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, United States |
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| Abstract: | Glucose oxidase (GOx) was immobilized onto glassy carbon electrode (GCE) that modified by reduced graphene oxide-gold nanoparticles- poly neutral red (RGO/AuNPs/PNR) nanocomposite. The composite was analyzed by scanning electron microscope (SEM), energy dispersive x-ray (EDX) spectroscopy, atomic force microscopy (AFM), attenuated total reflectance (ATR), cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). SEM/EDX analysis showed the morphological of the nanocomposite. AFM results showed the morphology and structure of the RGO/AuNPs and RGO surfaces. The covalent bonding between glucose oxidase and composite was confirmed by ATR technique. The electrochemical experiments were done in 100 mM phosphate buffer at pH 7 and temperature of 25 °C with three electrodes including Ag/AgCl, platinum wire and the modified GCE as the reference electrode, the auxiliary electrode and working electrode respectively. The electrochemical results confirmed the activity and direct electron transfer of immobilized enzyme. The immobilized electroactive GOx concentration was estimated 3.06 × 10−11 mol cm−2. The results showed the immobilized enzyme had a good stability and maintained 90% of its performance after two weeks. The nanocomposite bioanode in an air-birthing biofuel cell and 100 mM glucose concentration showed 176 μWcm−2 Power density. This strategy could be used for GOx-based biofuel cells. |
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| Keywords: | Direct electrochemistry Enzymatic biofuel cell Glucose oxidase Reduced graphene oxide Gold nanoparticles Poly neutral red |
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