Optimization studies to develop a low-cost medium for production of the lipases of Rhizopus microsporus by solid-state fermentation and scale-up of the process to a pilot packed-bed bioreactor |
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| Institution: | 1. Departamento de Engenharia Química, Universidade Estadual de Maringá, Avenida Colombo, 5790, Maringá 87020-900, Paraná, Brazil;2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil;3. Departamento de Química, Universidade Federal do Paraná, Cx. P. 19081 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil;1. Department of Microbiology, Faculty of Science, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok 10900, Thailand;2. Cassava and Starch Technology Research Laboratory, National Center for Genetic Engineering and Biotechnology, Pathum Thani 12120, Thailand;3. William G. Lowrie Department of Chemical and Biomolecular Engineering, Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA;1. Center of Natural Sciences, Federal University of São Carlos (UFSCar), Rodovia Lauri Simões de Barros km 12 (SP-189), 18290-000 Buri-SP, Brazil;2. Food Engineering and Technology Department, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto-SP, Brazil;3. Thermal Process Engineering/NaWiTec, Otto-von-Guericke University, Universitätsplatz 2, 39106 Magdeburg, Germany;1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba, Paraná 81531-980, Brazil;2. Departamento de Engenharia Química, Universidade Federal do Paraná, Cx. P. 19011 Centro Politécnico, Curitiba, Paraná 81531-980, Brazil;3. Departamento de Química, Universidade Federal do Paraná, Cx. P. 19081 Centro Politécnico, Curitiba, Paraná 81531-980, Brazil;1. Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuha?a 20, HR-31000 Osijek, Croatia;2. Group Micro Flow Chemistry and Process Technology, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands;3. School of Chemical Engineering, The University of Adelaide, Adelaide, 5005 South Australia, Australia |
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| Abstract: | A low-cost lipase preparation is required for enzymatic biodiesel synthesis. One possibility is to produce the lipase in solid-state fermentation (SSF) and then add the fermented solids (FS) directly to the reaction medium for biodiesel synthesis. In the current work, we scaled up the production of FS containing the lipases of Rhizopus microsporus. Initial experiments in flasks led to a low-cost medium containing wheat bran and sugarcane bagasse (50:50 w/w, dry basis), supplemented only with urea. We used this medium to scale-up production of FS, from 10 g in a laboratory column bioreactor to 15 kg in a pilot packed-bed bioreactor. This is the largest scale yet reported for lipase production in SSF. During scale-up, the hydrolytic activity of the FS decreased 57%: from 265 U g?1 at 18 h in the laboratory bioreactor to 113 U g?1 at 20 h in the pilot bioreactor. However, the esterification activity decreased by only 14%: from 12.1 U g?1 to 10.4 U g?1. When the FS produced in the laboratory and pilot bioreactors were dried and added directly to a solvent-free reaction medium to catalyze the esterification of oleic acid with ethanol, both gave the same ester content, 69% in 48 h. |
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| Keywords: | Biodiesel Lipases Solid-state fermentation Packed-bed bioreactor Scale-up |
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