Partial permeabilisation and depolarization of Salmonella enterica Typhimurium cells after treatment with pulsed electric fields and high pressure carbon dioxide |
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| Institution: | 1. Équipe TAI EA TIMR 4297, Université de Technologie de Compiègne, Centre de Recherche de Royallieu BP 20529-60205 Compiègne Cedex, France;2. Chemical Engineering Department, University of Balamand, Amioun, Lebanon;3. Équipe TAI EA TIMR 4297, École Supérieure de Chimie Organique et Minérale, 1 allée du réseau Jean-Marie Buckmaster 60200 Compiègne Cedex, France;4. Department of Physical Chemistry of Disperse Minerals, Institute of Biocolloidal Chemistry, NAS of Ukraine, 42, Blvr. Vernadskogo, Kyiv 03142, Ukraine;1. Laboratory of Bio-Nanotechnology, State Research Institute, Center for Physical Sciences and Technology, Sauletekio ave. 3, LT-10257 Vilnius, Lithuania;2. High Power Pulse Laboratory, State Research Institute, Center for Physical Sciences and Technology, Sauletekio ave. 3, LT-10257 Vilnius, Lithuania;3. State Research Institute Center for Innovative Medicine, Santari?ki? 5, LT-08406 Vilnius, Lithuania;4. Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, Sauletekio ave. 7, LT-10257 Vilnius, Lithuania |
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| Abstract: | The aim of the present study is to investigate the efficiency of the combined pulsed electric fields and high pressure carbon dioxide (PEF + HPCD) treatment on the Gram-negative pathogen Salmonella Typhimurium in a liquid medium, by means of both plate count technique and flow cytometry (FCM). PEF was applied at two conditions: (1) 1 single pulse of 1 ms length at 30 kV/cm and (2) 12 pulses of 4 ms length at 30 kV/cm, while HPCD at 12 MPa, 22 °C and 35 °C for different treating times (0–45 min). At both temperatures, the application of PEF as HPCD pre-treatment was demonstrated to enhance the inactivation kinetics and to decrease the treatment time to inactivate S. Typhimurium if compared to HPCD alone. Further, the approach based on FCM permitted to investigate the functional status of bacterial cells after PEF and HPCD treatments distinguishing among viable bacteria (considered as intact cells), permeabilised cells and depolarised cells simultaneously. It has been demonstrated that the synergistic effect is due to the electroporation effect of PEF which lead to changes in the cell membrane potential but also in a partial structural damage, favoring the subsequent CO2 penetration into the cells and increasing the inactivation kinetics, thus improving the efficiency of the entire process. |
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| Keywords: | High pressure carbon dioxide Pasteurization Pulsed electric fields Flow cytometry Depolarization |
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