Improvement of ganoderic acid production by fermentation of Ganoderma lucidum with cellulase as an elicitor |
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| Institution: | 1. School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore;2. State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo 25280, Coahuila, Mexico;2. Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, D.F., Mexico;3. Animal Nutrition Department, Universidad Autónoma Agraria Antonio Narro, Saltillo 25315, Coahuila, Mexico;1. Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, 34469 Maslak, Istanbul, Turkey;2. TUBITAK, Marmara Research Center Food Institute, 41470 Kocaeli, Turkey;1. College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing 210095, Jiangsu, People’s Republic of China;2. Department of Medical Technology, Medical College, Hebei University of Engineering, Handan 056038, Hebei, People’s Republic of China;1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China;2. Faculty of Science, Kunming University of Science and Technology, Kunming, 6500500, China |
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| Abstract: | Two-stage cultivation of Ganoderma lucidum was performed for the enhanced production of ganoderic acid (GA). Cellulase was identified to be an effective elicitor for the improvement of GA production, and GA titer reached 1334.5 mg/l compared to the control (779.6 mg/l) using lactose as the substrate without cellulase addition. Loading of 5 mg/l cellulase on day 3 resulted in the maximal GA titer of 1608 mg/l. To our knowledge, this is the first time that cellulase was used as the elicitor to enhance GA production. Submerged fermentation in a 2.0-l bioreactor was also conducted with cellulase as the elicitor, and as a result the maximal GA titer of 1252.7 mg/l was obtained on day 12. This is so far the best GA production obtained in submerged fermentation of G. lucidum. |
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| Keywords: | Ganoderic acid Two-stage fermentation Submerged fermentation Cellulase Elicitor |
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