Bioconversion with whole cell penicillin acylase in aqueous two-phase systems |
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Institution: | 2. Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan;1. Graduate School of Agriculture, Hokkaido University, Japan;2. Agricultural Radiation Research Center, National Agriculture and Food Research Organization, Japan;3. Faculty of Life and Environmental Sciences, Kyoto Prefectural University, Japan |
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Abstract: | The deacylation of Pen G was carried out by using recombinant E. coli in an aqueous two-phase system consisting of polyethylene glycol and potassium phosphate solution, which partitions the cells to the bottom phase and the products to the top phase. Bioconversion and product separation were carried out in the same reactor. Repeated batch conversion was employed ten times and enzymic activity showed only a slight decline. When pure enzyme was used for bioconversion in an aqueous two-phase system, the decline was fast and bioconversion using whole cell penicillin acylase was better than that obtained using the pure acylase. |
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