The use of glucose to regulate pH values of culture media and increase the production of baculovirus (BmNPV) and foreign protein (HBsAg) |
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| Institution: | 1. Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan;2. Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, MD 20742, USA;3. Department of Chemical Engineering, University of Maryland, College Park, MD 20742, USA;1. Janssen Research and Development, Turnhoutseweg 30, 2340 Beerse, Belgium;2. Janssen China Research & Development Center, 5F North Building #1 Jinchuang Mansion, 4560 Jinke Road, Shanghai 201210, China;1. School of Nursing, Li Ka Shing Faculty of Medicine, 21 Sassoon Road, Pokfulam, Hong Kong;2. College of Nursing & Health Innovation, Arizona State University, Phoenix, AZ, United States;1. Toronto Centre for Liver Disease, Toronto Western and General Hospital, University Health Network, University of Toronto, Toronto, Canada;3. Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands;4. Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany;6. Department of Medicine, Division of Gastroenterology, University of British Columbia, Vancouver, British Columbia, Canada;1. Department of Pediatric Hematology and Oncology, Hanover Medical School, Germany;2. Department of Pediatrics, ALL BFM trial center, University Medical Center Schleswig-Holstein, Campus Kiel, Germany;3. Department of Pediatric Hematology and Oncology, St. Anna Children''s Hospital, Medical University of Vienna, Austria;4. Department of Oncology, University Children''s Hospital, Zurich, Switzerland;1. Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;2. Microbiology and Immunology Department, Faculty of Pharmacy, Horus University, New Damietta, Egypt;3. Department of Endemic Hepatology and Gastroenterology, and Department of Tropical Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt;4. Biochemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;1. Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan;3. Hepatitis Research Center, National Taiwan University Hospital, Taipei, Taiwan |
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| Abstract: | When BmN-4 and M-BmN cells were grown in shake flasks, the pH initially dropped and later increased. The increase in pH signaled a ‘metabolic switch’ that was used here as an indicator for initiating a supplemental glucose and glutamine feed. Using the pH-based fed-batch culture method described, the maximum cell densities of BmN-4 cells and M-BmN cells were increased from 30×105 cells ml?1 to 43×105 and 52×105 cells ml?1, respectively. Correspondingly, the production of polyhedra (4·5×105 OBs ml?1) and HBsAg (574 ng ml?1), from the infection of BmN-4 and M-BmN by wild-type and recombinant BmNPV viruses, respectively, were both significantly enhanced 50% and 100%, respectively. This feeding strategy was implemented with no advanced instrumentation yet facilitated significantly increased yield in shake flasks. The technique should benefit those in research laboratories employing the baculovirus expression system as a rapid and efficient production system. |
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