Engineering of lipid microbubbles-coated copper and selenium nanoparticles: Ultrasound-stimulated radiation of anticancer activity ian human ovarian cancer cells |
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| Institution: | 1. Ultrasonography Department, People’s Hospital of Rongcheng, Rongcheng, 264300, China;2. Department of Gynecology, the First People''s Hospital of Jining City 272000, China;3. Department of Gynecology, Binzhou Hospital of Traditional Chinese Medicine, Binzhou, 256600, China;4. Ultrasonography Department, Linyi People''s Hospital, Linyi, 276000, China;1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;2. School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;1. Department of Mechanical and Electro-Mechanical Engineering, National I-Lan University, Yilan, Taiwan;2. Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia;3. Centre For Automotive Research and Tribology, Indian Institute of Technology, Delhi, India;4. Department of Mechanical Engineering, and Center for Engineering and Sustainable Development Research, De La Salle University, Philippines;5. School of Information, Systems and Modelling, Faculty of Engineering and Information Technology, University of Technology Sydney, NSW 2007, Australia;1. Sol-Gel Division, CSIR-Central Glass and Ceramic Research Institute, 196 Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India;2. Cell Biology & Physiology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India;1. Food College, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, PR China;2. College of Information Science and Technology, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, PR China |
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| Abstract: | In this study, we have designed developed the microbubbles (MBs) coated Cu-Se nanoparticles (MBs@Cu-Se NPs) was efficiently synthesized and combined with the Ultrasound for potential anticancer effects in ovarian carcinoma cells. The fabricated nanoparticles are perceived as the well-known spherical size by SEM and TEM analysis. The hydrodynamic parameters of the MBs@Cu-Se NPs confirm via DLA analysis. Further, in vitro cancer potential of the Cu-Se, MBs@Cu-Se NPs and MBs@Cu-Se NPs with Ultrasound efficiently kills the ovarian cancer cells (A2780 and CisRA2780) and reduced the cell viability in a dose-dependent manner. The MBs@Cu-Se NPs with Ultrasound displayed remarkably higher amounts of apoptosis of the ovarian cancers, which was confirmed by the various biochemical assays (AO-EB), and Hoechst-33,258 by nuclear staining. Additionally, we examined the apoptosis mechanism through the flow cytometry technique. The results reveal that the MBs@Cu-Se NPs with Ultrasound significantly induce apoptosis in both cancer cells. These results suggest that the in vitro cytotoxicity potential of MBs@Cu-Se NPs with Ultrasound combination therapy against ovarian carcinoma. Overall, new approaches of combination therapy of MBs@Cu-Se NPs with Ultrasound could be a promising alternative strategy and efficient chemotherapy as well as radiotherapy. |
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| Keywords: | Copper and selenium nanoparticles Lipid microbubbles Ultrasound Ovarian cancer Apoptosis |
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